Exploration of central dopamine transporter and D2 receptor in morphine abstinent rats

LIN Yansong1 DING Shiyu2 CHEN Zhengping2 WANG Bocheng1 ZHANG Manda2 WANG Shizhen1 1

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Exploration of central dopamine transporter and D2 receptor in morphine abstinent rats

更新时间：2021-03-12

- Exploration of central dopamine transporter and D2 receptor in morphine abstinent rats
- NUCLEAR TECHNIQUES Issue 1, (2006)
- 作者机构：
  
  1. 北京协和医院核医学科
  2. 江苏省原子医学研究所核医学国家重点实验室
  3. 北京协和医院核医学科,北京,100730
  4. ,无锡,214063
- 作者简介：
- 基金信息：
  
  国家自然基金资助(项目批准号30100044)项目
- DOI：
  CLC： R749.61
- Published：01 January 2006
- 稿件说明：
移动端阅览
[1]林岩松,丁时禹,陈正平,王博诚,张满达,王世真.吗啡戒断大鼠脑多巴胺转运蛋白及D2受体的研究[J].核技术,2006(01):55-58.

LIN Yansong1 DING Shiyu2 CHEN Zhengping2 WANG Bocheng1 ZHANG Manda2 WANG Shizhen1 1. Exploration of central dopamine transporter and D2 receptor in morphine abstinent rats[J]. Nuclear techniques, 2006, (1): 55-58.
[1]林岩松,丁时禹,陈正平,王博诚,张满达,王世真.吗啡戒断大鼠脑多巴胺转运蛋白及D2受体的研究[J].核技术,2006(01):55-58. DOI：

LIN Yansong1 DING Shiyu2 CHEN Zhengping2 WANG Bocheng1 ZHANG Manda2 WANG Shizhen1 1. Exploration of central dopamine transporter and D2 receptor in morphine abstinent rats[J]. Nuclear techniques, 2006, (1): 55-58. DOI：

摘要

采用多巴胺转运蛋白（DAT）示踪剂125I-甲基-3β-（4-碘苯基）托烷-2β-羧酸酯（β-CIT）及D2受体示踪剂125I-左旋-3-碘-2-羟基-6-甲氧基-N[（1-乙基-2-吡咯烷）甲基]苯酰胺（IBZM）探讨吗啡戒断前、后大鼠脑突触前、后多巴胺（DA）系统的变化。吗啡戒断1、2、3天组大鼠（各10只）分别于连续给予吗啡（20mg/kg）8天后停止给予吗啡1、2、3天再进行实验;吗啡（20mg/kg）组及生理盐水对照组大鼠各12只;对照组大鼠只给予腹腔注射0.3mL生理盐水

共计8天。将吗啡组、戒断1、2、3天组及生理盐水对照组大鼠各进一步随机平均分为两组

分别用于125I-IBZM、125I-β-CIT脑内分布研究。结果:（1）吗啡戒断组大鼠自戒断第2天开始出现明显的腹泻症状

同时还伴有叩齿及寒战等症状出现。（2）在20mg/kg吗啡及戒断组的125I-β-CIT脑内分布中

吗啡组在纹状体（ST）、伏隔核（NAC）的分布明显高于戒断1、2、3天组和对照组（P<0.05）

在额叶（FC）、海马（HIP）的分布也高于戒断组及对照组（P<0.05）。戒断1、2、3天组在ST、NAC及HIP的分布与对照组比较差异无显著性（P>0.05）。（3）125I-IBZM在吗啡依赖及戒断组的脑内分布显示:吗啡组在ST、NAC的分布明显低于戒断1、2、3天组和对照组（P<0.05）;在HIP及皮层的分布也低于对照组及戒断各组（P<0.05）。戒断1、2、3天组在ST、NAC的125I-IBZM分布增加逐渐增高

其中戒断各组在ST的分布均低于对照组（P<0.05）;在NAC戒断1天组仍低于对照组（P<0.05）

而戒断2、3天组125I-IBZM在NAC的分布与对照组比较差异无显著性（P>0.05）。由此可得出结论:吗啡戒断组大鼠出现了明显的戒断症状。在吗啡依赖中ST、NAC及HIP等的DAT出现了上调

D2受体则出现一种下调的低敏状态

吗啡戒断使这种增高DA能的活动及DAT回落并趋于正常范围

并使NAC及ST下调的D2受体逐渐回升。

Abstract

The experiment was designed to investigate the variation of DAT and D2 receptor in morphine administered and 1

3 day abstinent rats. Morphine exposure was induced by repeated morphine (i.p.) treatment for 8 days. Conditioned place preference test was conducted to evaluate the drug seeking behaviour and morphine dependence of rats with morphine exposure. Biodistribution of the imaging agents 125I-β-CIT and 125I-IBZM was used to evaluate the central DAT and D2 receptor during morphine exposure and 1

3 day’s abstinence. Results reveal the following facts. (1) The morphine abstinent rats showed diarrhea and body-shake 1 day after morphine withdrawal. (2) For morphine group

125I-β-CIT %ID/g in ST and NAC was higher than that of the 1

3 day’s abstinent rats and control (P<0.05). No difference was found between 1

3 day’s abstinent group and control group (P>0.05). (3) 125I-IBZM %ID/g in ST

NAC and HIP in morphine rats were lower than those of the abstinent and control rats (P<0.05). The 125I-IBZM %ID/g in ST and NAC gradually increased with the abstinent days. While in ST the %ID/g among the abstinent rats was all lower than that of the control rats

in NAC the %ID/g was still lower in 1 day’s abstinent rats (P<0.05)

and no difference in 2 and 3 day’s abstinent rats was observed in comparison with the control (P>0.05)

indicating the reduction of hyper-activated DAT and the increase of down-regulatory D2 receptor induced by morphine during morphine withdrawal. Our results confirmed that the dopamine system

especially DAT and D2 receptor in mesolimbic and meso-striatum pathway

has been implicated in morphine treatment. The rewarding properties of morphine and the somatic expression of morphine abstinence were related to changes in mesolimbic and meso-striatum dopaminergic activity.

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