The effects of non-implantation factors

such as drying

vacuum and the staying time of the E.coli LE392 culture

on survival rate of E.coli LE392 were studied when E.coli LE392 was irradiated by the low-energy N+. The results show that the survival rate of E.coli LE392 does not reduce steadily all the time but rapidly drops sometime during drying. The survival rate of E.coli LE392 declines sharply as the samples are placed in vacuum

then falls in- distinctively with increasing of time. The tolerance of E.coli LE392 towards vacuum increasingly strengthens when the E.coli LE392 culture is placed at room temperature. Preparing the culture in batchs can ensure the consistency of the irradiated samples and avoid errors caused by the inconsistent samples. When the non-implantation factors are controlled

E.coli is implanted by 30 keV N+ of 1×1014 cm-2 and 3×1015 cm-2

respectively. And the results show no difference in the E.coli’s survival rates between batchs at the same dose.