Studies of DNA damage induced by synchrotron radiation soft X-rays using single cell gel electrophoresis

GU Yuehua GAO Hengjing WANG Qi ZHAO Ling

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Studies of DNA damage induced by synchrotron radiation soft X-rays using single cell gel electrophoresis

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- Studies of DNA damage induced by synchrotron radiation soft X-rays using single cell gel electrophoresis
- NUCLEAR TECHNIQUES Issue 7, (2006)
- 作者机构：
  
  1. 中国科学技术大学生命科学学院
  2. 中国科学技术大学生命科学学院,合肥,230027
- 作者简介：
- 基金信息：
- DOI：
  CLC： Q691
- Published：01 July 2006
- 稿件说明：
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[1]顾月华,高恒景,王琦,赵凌.单细胞凝胶电泳法检测研究同步辐射软X射线辐照引起的DNA损伤[J].核技术,2006(07):481-484.

GU Yuehua GAO Hengjing WANG Qi ZHAO Ling. Studies of DNA damage induced by synchrotron radiation soft X-rays using single cell gel electrophoresis[J]. Nuclear techniques, 2006, (7): 481-484.
[1]顾月华,高恒景,王琦,赵凌.单细胞凝胶电泳法检测研究同步辐射软X射线辐照引起的DNA损伤[J].核技术,2006(07):481-484. DOI：

GU Yuehua GAO Hengjing WANG Qi ZHAO Ling. Studies of DNA damage induced by synchrotron radiation soft X-rays using single cell gel electrophoresis[J]. Nuclear techniques, 2006, (7): 481-484. DOI：

摘要

本实验采用单细胞凝胶电泳方法定量检测了不同辐照剂量的同步辐射软X射线对小麦根尖细胞造成的DNA单链损伤。通过测定核DNA的迁移效应表明

在剂量为0—288J·cm-2范围内的软X射线辐照下

有20%—92%的小麦根尖细胞产生了DNA单链的损伤效应

同时DNA的损伤程度也与辐照剂量呈显著正相关。

Abstract

The DNA damage of wheat root tip cells induced by different doses (0—288 J·cm-2) of synchrotron radiation (SR) soft X-rays was measured by using the single-cell gel electrophoresis (SCGE) technique. The DNA removal in electrophoresis was observed and the results showed that 20%—92% of wheat root tip cells were damaged with the increase of radiation dose

and the DNA damage level (expressed by Tail Moment) was positively correlated with the dose of synchrotron radiation soft X-rays.

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