Radiosensitivity and cell cycle of HepG2 hepatoma cell line irradiated by ionizing radiation with different linear energy transfer

WEI Wei1; 3 LI Wenjian1 XIA Jingguang1; 3 JIN Xiaodong1 GUO Chuanling1; 3 SU Xu2 1

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Radiosensitivity and cell cycle of HepG2 hepatoma cell line irradiated by ionizing radiation with different linear energy transfer

更新时间：2021-03-12

- Radiosensitivity and cell cycle of HepG2 hepatoma cell line irradiated by ionizing radiation with different linear energy transfer
- NUCLEAR TECHNIQUES Issue 9, (2006)
- 作者机构：
  
  1. 中国科学院近代物理研究所
  2. 中国疾病预防控制中心辐射防护与核安全医学所
- 作者简介：
- 基金信息：
  
  国家自然科学基金重点项目(10335050);科技部重大基础研究前期研究专项资助项目(2003CCB00200)
- DOI：
  CLC： R735.7
- Published：01 September 2006
- 稿件说明：
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[1]魏巍,李文建,夏景光,金晓东,郭传玲,苏旭.不同LET辐射对HepG2肝癌细胞的辐射敏感性与周期阻滞的影响[J].核技术,2006(09):679-684.

WEI Wei1, 3 LI Wenjian1 XIA Jingguang1, 3 JIN Xiaodong1 GUO Chuanling1, et al. Radiosensitivity and cell cycle of HepG2 hepatoma cell line irradiated by ionizing radiation with different linear energy transfer[J]. Nuclear techniques, 2006, (9): 679-684.
[1]魏巍,李文建,夏景光,金晓东,郭传玲,苏旭.不同LET辐射对HepG2肝癌细胞的辐射敏感性与周期阻滞的影响[J].核技术,2006(09):679-684. DOI：

WEI Wei1, 3 LI Wenjian1 XIA Jingguang1, 3 JIN Xiaodong1 GUO Chuanling1, et al. Radiosensitivity and cell cycle of HepG2 hepatoma cell line irradiated by ionizing radiation with different linear energy transfer[J]. Nuclear techniques, 2006, (9): 679-684. DOI：

摘要

本工作研究不同LET射线辐照对HepG2肝癌细胞辐射敏感性、周期进程和凋亡的影响

为重离子治疗癌症的临床应用积累基础数据。以0、0.5、1、2、4、8Gy剂量的12C6+离子及X射线分别照射处于指数生长期的HepG2细胞

用克隆形成率测定细胞辐射敏感性

通过流式细胞术测定细胞DNA含量以确定各时相细胞的比例及细胞凋亡情况。实验结果显示

12C6+离子辐照所致的HepG2细胞存活率明显低于X射线。随着吸收剂量的增加和修复时间的延长

12C6+离子能导致更显著的细胞S期阻滞、G2/M期阻滞延迟和细胞凋亡。说明与X射线相比

12C6+离子辐照能更有效地杀伤HepG2肝癌细胞并诱导其凋亡。

Abstract

In this paper

the effect of the ionizing radiation with different linear energy transfer on radiosensitivity

cell cycle alteration and the change of apoptosis in HepG2 hepatoma cell lines are studied to build up the base data for clinical therapy. Exponentially growing HepG2 cell lines are irradiated by 12C6+ ions and X-ray at the doses of 0

0.5

4and 8Gy

respectivity. The radiosensitivity is assessed by means of the colony-forming assay. The DNA content

percentage of each cell-cycle phase and the apoptosis rate are obtained with the flow cytometry methods. The results indicate that compared with X-ray

the survival fraction of HepG2 hepatoma cells irradiated by 12C6+ ions is evidently lower

the S phase arrest

G2/M phase arrest delay and the apoptosis in HepG2 cells irradiated by 12C6+ ions are more prominent with the increase of dose and repaired time

thus suggesting that 12C6+ ions can more effectively kill the HepG2 cells and induce apoptosis in the cells than X-ray.

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XIA Jingguang1

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