Study on protection from Arabidopsis callus hazard in ion beam implantation process

GU Yunhong1; 2 YU Zengliang1 QIN Guangyong2 HUO Yuping2          1

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Study on protection from Arabidopsis callus hazard in ion beam implantation process

更新时间：2021-03-12

- Study on protection from Arabidopsis callus hazard in ion beam implantation process
- NUCLEAR TECHNIQUES Issue 2, (2004)
- 作者机构：
  
  1. 中国科学院等离子体物理研究所离子束生物工程学重点实验室,中国科学院等离子体物理研究所离子束生物工程学重点实验室,郑州大学离子束生物工程省重点实验室,郑州大学离子束生物工程省重点实验室,合肥,230031
  2. 郑州大学离子束生物工程省重点实验室郑州450052,合肥230031,郑州450052,郑州,450052
- 作者简介：
- 基金信息：
- DOI：
  CLC： S426
- Published：01 February 2004
- 稿件说明：
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[1]谷运红,余增亮,秦广雍,霍裕平.离子注入过程中拟南芥愈伤组织冻害的防护研究[J].核技术,2004(02):112-115.

GU Yunhong1, 2 YU Zengliang1 QIN Guangyong2 HUO Yuping2 1. Study on protection from Arabidopsis callus hazard in ion beam implantation process[J]. Nuclear techniques, 2004, (2): 112-115.
[1]谷运红,余增亮,秦广雍,霍裕平.离子注入过程中拟南芥愈伤组织冻害的防护研究[J].核技术,2004(02):112-115. DOI：

GU Yunhong1, 2 YU Zengliang1 QIN Guangyong2 HUO Yuping2 1. Study on protection from Arabidopsis callus hazard in ion beam implantation process[J]. Nuclear techniques, 2004, (2): 112-115. DOI：

摘要

以拟南芥愈伤组织为对象，探讨了 DMSO（二甲亚砜）处理和玻璃化处理两种方法对愈伤组织在注入过程中冻害的防护。结果表明，玻璃化处理较 DMSO 处理能更有效地对冻害进行防护，N+注入量增至200×2.6×1013cm? 时，仍有 60%左右的愈伤组织可以正常分化。此工作对于以活体材料为注入对象的研究有一定的实际意义。

Abstract

The protection effect of DMSO and vitrification from Arabidopsis callus hazard was studied in the process of ion beam implantation. It was found that the protection effect of vitrification was higher than that of DMSO. When the implanted N+dose was increased to 200×2.6×1013cm?

survival rate of Arabidopsis callus was 2 about 60%. The result has some significance to the ion implantation research of in-vitro material.

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