The SRXRF analysis of trace elements in Cu-binding proteins separated from Elsholtzia splendens roots

SHI Jiyan1 CHEN Yingxu1 YUAN Xiaofeng3 WU Bei1 HUANG Yuying2 HE Wei2                              1

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The SRXRF analysis of trace elements in Cu-binding proteins separated from Elsholtzia splendens roots

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- The SRXRF analysis of trace elements in Cu-binding proteins separated from Elsholtzia splendens roots
- NUCLEAR TECHNIQUES Issue 10, (2004)
- 作者机构：
  
  1. 浙江大学环资学院环境工程系
  2. 浙江中医学院生命科学系
  3. 中国科学院高能物理研究所,杭州,310029
  4. ,北京,100039
- 作者简介：
- 基金信息：
  
  国家自然科学基金项目(40271060);国家 863 项目(2001AA645010);浙江省自然科学基金项目(402017)资助
- DOI：
  CLC： X173
- Published：01 October 2004
- 稿件说明：
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[1]施积炎,陈英旭,袁小凤,武贝,黄宇营,何伟.同步辐射X荧光分析海州香薷根中铜结合蛋白的微量元素[J].核技术,2004(10):736-739.

SHI Jiyan1 CHEN Yingxu1 YUAN Xiaofeng3 WU Bei1 HUANG Yuying2 HE Wei2 1. The SRXRF analysis of trace elements in Cu-binding proteins separated from Elsholtzia splendens roots[J]. Nuclear techniques, 2004, (10): 736-739.
[1]施积炎,陈英旭,袁小凤,武贝,黄宇营,何伟.同步辐射X荧光分析海州香薷根中铜结合蛋白的微量元素[J].核技术,2004(10):736-739. DOI：

SHI Jiyan1 CHEN Yingxu1 YUAN Xiaofeng3 WU Bei1 HUANG Yuying2 HE Wei2 1. The SRXRF analysis of trace elements in Cu-binding proteins separated from Elsholtzia splendens roots[J]. Nuclear techniques, 2004, (10): 736-739. DOI：

摘要

利用 Sephadex G-50 凝胶过滤从 Cu 处理海州香薷根中分离得到两个不同的铜结合蛋白。用同步辐射 X荧光（SRXRF）分析了两个蛋白的微量元素

结果表明分子量较小的铜结合蛋白 2 与 Cu 络合能力要强于分子量较大的铜结合蛋白 1。另外

Pb、Fe 和 Zn 等金属元素可以与铜结合蛋白竞争结合。两个铜结合蛋白中 S 含量都很低

说明海州香薷根中铜结合蛋白可能并不以含硫量较高的金属硫蛋白（MTs）或植物络合肽（PCs）为主。实验证明 SRXRF 技术可以很好地用于凝胶过滤分离蛋白的元素分析。

Abstract

Through the gel filtration chromatography on Sephadex G-50

two different Cu-binding proteins were separated from copper treated Elsholtzia splendens roots. The elements distribution in the two Cu-binding proteins was investigated by SRXRF. The results showed that the Cu-chelate ability of Cu-binding protein 2 is better than that of Cu-binding protein 1. Besides

and Zn can compete with Cu to chelate the Cu-binding proteins. The content of element S is very low in the two Cu-binding proteins

which demonstrates that MTs or PCs are not dominant in the Cu-binding proteins in Elsholtzia splendens roots. The experiment also has proved that SRXRF can be used to study elements distribution in the protein separated by gel filtration chromatography.

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