Investigation of metalloproteins distributions in cytosol of hepatocellular carcinoma and its surrounding tissues by using synchrotron radiation X-ray fluorescence

GAO Yuxi~1 CHEN Chunying~1 LI Bai~1 HUANG Yuying~2 HE Wei~2 DENG Guilong~3 LIU Yingbin~3 CHAI Zhifang~1 1

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Investigation of metalloproteins distributions in cytosol of hepatocellular carcinoma and its surrounding tissues by using synchrotron radiation X-ray fluorescence

更新时间：2021-03-12

- Investigation of metalloproteins distributions in cytosol of hepatocellular carcinoma and its surrounding tissues by using synchrotron radiation X-ray fluorescence
- NUCLEAR TECHNIQUES Issue 12, (2004)
- 作者机构：
  
  1. 中国科学院高能物理研究所核分析技术重点实验室
  2. 中国科学院高能物理研究所同步辐射国家实验室
  3. 浙江大学第二附属医院普外科
  4. 中国科学院高能物理研究所核分析技术重点实验室,北京,100049
  5. ,杭州,310009
- 作者简介：
- 基金信息：
  
  国家自然科学基金(10475092,10490180);中国科学院重大项目(KJCX2-N01)资助
- DOI：
  CLC： R735.7
- Published：01 December 2004
- 稿件说明：
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[1]高愈希,陈春英,李柏,黄宇营,何伟,邓贵龙,刘颖斌,柴之芳.人肝癌及癌旁组织细胞胞质溶胶中金属蛋白分布的同步辐射X荧光分析研究[J].核技术,2004(12):963-967.

GAO Yuxi~1 CHEN Chunying~1 LI Bai~1 HUANG Yuying~2 HE Wei~2 DENG Guilong~3 LIU Yingbin~3 CHAI Zhifang~1 1. Investigation of metalloproteins distributions in cytosol of hepatocellular carcinoma and its surrounding tissues by using synchrotron radiation X-ray fluorescence[J]. Nuclear techniques, 2004, (12): 963-967.
[1]高愈希,陈春英,李柏,黄宇营,何伟,邓贵龙,刘颖斌,柴之芳.人肝癌及癌旁组织细胞胞质溶胶中金属蛋白分布的同步辐射X荧光分析研究[J].核技术,2004(12):963-967. DOI：

GAO Yuxi~1 CHEN Chunying~1 LI Bai~1 HUANG Yuying~2 HE Wei~2 DENG Guilong~3 LIU Yingbin~3 CHAI Zhifang~1 1. Investigation of metalloproteins distributions in cytosol of hepatocellular carcinoma and its surrounding tissues by using synchrotron radiation X-ray fluorescence[J]. Nuclear techniques, 2004, (12): 963-967. DOI：

摘要

肝癌发生与组织中微量元素水平和一些酶的活性相关。为研究肝癌组织内金属蛋白的分布特征并寻找有意义的微量元素存在形式加以深入研究

用等电聚焦（IEF）分离肝细胞癌以及癌旁组织细胞胞质溶胶内的蛋白质

用同步辐射X荧光分析蛋白条带内的金属含量。初步研究表明:在肝细胞癌和癌旁组织的细胞胞质溶胶中所检出的含铁、铜、锌蛋白基本是一致的

一般癌旁组织蛋白质的铁、铜、锌含量高于癌组织

癌旁组织中铁含量高主要体现在pI为6.5、7.7、8.0及小于3.5的含铁条带内

而癌组织中pI为4.0、7.0的条带内铁含量略高于癌旁组织

癌旁组织的pl为3.2、4.9、5.5、5.9和6.5的条带铜含量明显高于癌组织

锌含量的升高则主要体现在pI为5.5和6.5的含锌条带内

这些分布特征的普遍性及生物学意义有待进一步研究。

Abstract

Synchrotron radiation X-ray fluorescence(SRXRF) spectroscopy is an advanced quantitative multielemental analytical technique with space resolution of several μm and sensitivities in the μg/g range. It can be used for keeping track of trace elements in biological samples after an electrophoretic separation. In this paper

proteins in cytosol of human hepatocellular carcinoma and the surrounding "normal" tissue were separated with thin layer isoelectric focusing(IEF). The contents of metal ions in protein bands were determined by SRXRF. The results showed that the metal-containing proteins detected in the two samples were very much alike

but their distribution patterns were easily distinguishable. The contents of iron

zine

and copper in bands from the surrounding "normal" tissue were generally higher than that from hepatoma tissue

especially in Fe-eontaining proteins with pls of 6.5

7.7

8.0 and less than 3.5

Cu-containing proteins with pls of 3.2

4.9

5.5

5.9

and 6.5

as well as Zn-containing proteins with pI of 5.5 and 6.5. However

Fe contents in Fe-containing proteins of 4.0

and 7.0 from the hepatoma tissue were slight higher than that from the surrounding "normal" tissue. Further studies are necessary to validate the universality and the biological meaning of the pattern.

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Related Author

柴之芳

黄宇营

何伟

孙瑾

李柏

陈春英

2 GAO Yuxi 1 CHEN Chunying1 LI Bai1 SUN Jin1 HE Wei1 HUANG Yuying1 CHAI Zhifang1 1

DONG Yuanxing1

Related Institution

Key Laboratory of Nuclear Analytical Techniques, Institute of High Energy Physics, the Chinese Academy of Sciences, Beijing 100049,China) 2

中国科学院高能物理研究所核分析技术重点实验室北京100049忻州师范学院物理系忻州

Laboratory of Nuclear Analysis Techniques, Institute of High Energy Physics, the Chinese Academy of Sciences, Beijing 100039) 2

Laboratory of Nuclear Analytical Techniques, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100039) 2

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