Determination of chromium combinated with DNA,RNA and protein in chromium-rich brewer’s yeast

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Determination of chromium combinated with DNA,RNA and protein in chromium-rich brewer’s yeast

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- Determination of chromium combinated with DNA,RNA and protein in chromium-rich brewer’s yeast
- NUCLEAR TECHNIQUES Issue 4, (2000)
- 作者机构：
  
  1. 中国科学院高能物理研究所核分析技术开放实验室,北京,100080
  2. 中国科学院高能物理研究所核分析
- 作者简介：
- 基金信息：
  
  国家自然科学基金“八五”重大项目!19392100;中国科学院重大项目!21039751
- DOI：
  CLC： Q78
- Published：01 April 2000
- 稿件说明：
移动端阅览
[1]丁文军,钱琴芳,侯小琳,丰伟悦,柴之芳,王珂.富铬酵母细胞的DNA、RNA和蛋白质结合铬的测定[J].核技术,2000(04):231-235.

Determination of chromium combinated with DNA,RNA and protein in chromium-rich brewer’s yeast[J]. Nuclear techniques, 2000, (4): 231-235.
[1]丁文军,钱琴芳,侯小琳,丰伟悦,柴之芳,王珂.富铬酵母细胞的DNA、RNA和蛋白质结合铬的测定[J].核技术,2000(04):231-235. DOI：

Determination of chromium combinated with DNA,RNA and protein in chromium-rich brewer’s yeast[J]. Nuclear techniques, 2000, (4): 231-235. DOI：

摘要

使用中子活化分析方法测定了富铬酵母与普通酵母的ＤＮＡ、ＲＮＡ和蛋白质中铬的含量，研究铬与ＤＮＡ、ＲＮＡ和蛋白质的结合情况。结果发现，两种酵母的ＤＮＡ、ＲＮＡ和蛋白质提取率和浓度无明显差异，但富铬酵母中ＤＮＡ、ＲＮＡ和蛋白质的铬含量高于普通酵母，而且铬与ＤＮＡ的结合量明显高于ＲＮＡ和蛋白质，说明酵母细胞在含铬的培养基中培养时，铬进入细胞体内产生生物转化，并与酵母细胞的ＤＮＡ、ＲＮＡ和蛋白质发生了结合。

Abstract

The contents of chromium in the DNA

RNA and protein fractions separated from chromium-rich and normal brewer’s yeast were determined with the neutron activation analysis in order to study the combination of Cr with DNA

RNA and protein in chromium-rich brewer’s yeast. Our results showed that the extracting rats and concentrations of DNA

RNA and protein had no significant difference in two types of yeast

but the chromium contents of DNA

RNA and protein in the chromium-rich yeast were significantly higher than those in the normal. In addition

the content of chromium in DNA was much higher than that in RNA and protein

which indicated that the inorganic chromium compounds entered into the yeast cell

during the yeast cultivation in the culture medium containing chromium were converted into organic chromium compounds combined with DNA

RNA and protein.

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