Analysis of zinc in protein bands after electrophoresis separation by synchrotron radiation  X-ray fluorescence

GAO Yuxi1 FENG Weiyue1 LI Bai1 ZHANG Peiqun1 HE Wei2 HUANG Yuying2 CHAI Zhifang1         1

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Analysis of zinc in protein bands after electrophoresis separation by synchrotron radiation X-ray fluorescence

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- Analysis of zinc in protein bands after electrophoresis separation by synchrotron radiation X-ray fluorescence
- NUCLEAR TECHNIQUES Issue 3, (2004)
- 作者机构：
  
  1. 中国科学院高能物理研究所核分析技术重点实验室
  2. 中国科学院高能物理研究所同步辐射国家实验室
  3. 中国科学院高能物理研究所核分析技术重点实验室,北京,100039
- 作者简介：
- 基金信息：
  
  国家自然科学基金 (No.20071032);中国科学院重大项目 ( KJCX2-N01 )资助课题
- DOI：
  CLC： Q51
- Published：01 March 2004
- 稿件说明：
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[1]高愈希,丰伟悦,李柏,章佩群,何伟,黄宇营,柴之芳.同步辐射X荧光法测定电泳分离后蛋白条带内的锌[J].核技术,2004(03):165-168.

GAO Yuxi1 FENG Weiyue1 LI Bai1 ZHANG Peiqun1 HE Wei2 HUANG Yuying2 CHAI Zhifang1 1. Analysis of zinc in protein bands after electrophoresis separation by synchrotron radiation X-ray fluorescence[J]. Nuclear techniques, 2004, (3): 165-168.
[1]高愈希,丰伟悦,李柏,章佩群,何伟,黄宇营,柴之芳.同步辐射X荧光法测定电泳分离后蛋白条带内的锌[J].核技术,2004(03):165-168. DOI：

GAO Yuxi1 FENG Weiyue1 LI Bai1 ZHANG Peiqun1 HE Wei2 HUANG Yuying2 CHAI Zhifang1 1. Analysis of zinc in protein bands after electrophoresis separation by synchrotron radiation X-ray fluorescence[J]. Nuclear techniques, 2004, (3): 165-168. DOI：

摘要

本文初步建立了一种基于电泳分离和同步辐射X荧光（SRXRF）分析技术的生物样品内微量元素的种态分析方法。用同步辐射X荧光法直接分析了人肝细胞胞质溶胶等电聚焦（IEF）分离后蛋白条带内的金属锌含量。结果表明:肝细胞胞质溶胶内主要有三个含锌条带

等电点（pI）分别为4.69、5.93、9.84。含锌量分别占总检出锌的33.08%、17.05%、26.54%。其中等电点为4.69的蛋白可能为金属硫蛋白。另外在等电点为6.60—9.30的范围内有若干含锌蛋白

共占总检出锌的23.33%。

Abstract

A procedure based on isoelectric focusing ( IEF ) separation and synchrotron radiation X-ray fluorescence ( SRXRF ) analysis for speciation of trace elements in biological samples was preliminarily established. The proteins in human liver cytosol were separated by IEF

and zinc amount in each protein band was measured by SRXRF. The results indicate that there were three main zinc-containing bands with isoelectric points ( pIs) of 4.69

5.93

and 9.84 present in the sample

and the zinc percentages in the three bands to the total recovered zinc were 33.08%

17.05%

and 26.54%

respectively. In addition

several zinc-containing proteins were present in the pI range of 6.6 ? 9.3

which amounted to 23.33 % of the recovered zinc.

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Related Author

DONG Yuanxing1

2 GAO Yuxi 1 CHEN Chunying1 LI Bai1 SUN Jin1 HE Wei1 HUANG Yuying1 CHAI Zhifang1 1

陈春英

李柏

孙瑾

何伟

黄宇营

柴之芳

Related Institution

Key Laboratory of Nuclear Analytical Techniques, Institute of High Energy Physics, the Chinese Academy of Sciences, Beijing 100049,China) 2

中国科学院高能物理研究所核分析技术重点实验室北京100049忻州师范学院物理系忻州

Key Laboratory of Nuclear Analytical Techniques, Institute of High Energy Physics, the Chinese Academy of Sciences, Beijing 100049) 2

浙江大学第二附属医院普外科

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