A study of 99Tcm labelling of monoclonal antibody 2F7F（ab′）2 segment against small cell lung cancer

HUANG Biao1; 2 ZHANG Xiangrui1 ZHU lan1 MO Zhonggen3 YANG Yaoqin3 ZHAO Xintai3   1

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A study of ⁹⁹Tc^m labelling of monoclonal antibody 2F7F_（ab′）2 segment against small cell lung cancer

更新时间：2021-03-12

- A study of ⁹⁹Tc^m labelling of monoclonal antibody 2F7F_（ab′）2 segment against small cell lung cancer
- NUCLEAR TECHNIQUES Issue 5, (2004)
- 作者机构：
  
  1. 江苏省原子医学研究所,江苏省原子医学研究所,江苏省原子医学研究所,上海市肿瘤研究所,上海市肿瘤研究所,上海市肿瘤研究所,无锡,214063
  2. 江南大学生物工程学院无锡214036,无锡214063,无锡214063,上海200032,上海200032,上海,200032
- 作者简介：
- 基金信息：
  
  国家 863 计划资助(2001AAA215141)申请国家一类新药(西药申临字第 97190 号)
- DOI：
  CLC： R734.2
- Published：01 May 2004
- 稿件说明：
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[1]黄飚,张祥瑞,朱岚,莫忠根,杨瑶琴,赵新泰.小细胞肺癌单克隆抗体2F7F_((ab′)2)片段的~(99)Tc~m标记试剂盒的制备[J].核技术,2004(05):354-356.

HUANG Biao1, 2 ZHANG Xiangrui1 ZHU lan1 MO Zhonggen3 YANG Yaoqin3 ZHAO Xintai3 1. A study of ⁹⁹Tc^m labelling of monoclonal antibody 2F7F_（ab′）2 segment against small cell lung cancer[J]. Nuclear techniques, 2004, (5): 354-356.
[1]黄飚,张祥瑞,朱岚,莫忠根,杨瑶琴,赵新泰.小细胞肺癌单克隆抗体2F7F_((ab′)2)片段的~(99)Tc~m标记试剂盒的制备[J].核技术,2004(05):354-356. DOI：

HUANG Biao1, 2 ZHANG Xiangrui1 ZHU lan1 MO Zhonggen3 YANG Yaoqin3 ZHAO Xintai3 1. A study of ⁹⁹Tc^m labelling of monoclonal antibody 2F7F_（ab′）2 segment against small cell lung cancer[J]. Nuclear techniques, 2004, (5): 354-356. DOI：

摘要

采用2-亚氨噻酚盐酸盐修饰2F7F（ab′）2

通过葡庚糖酸转换

将Tcm标记在小细胞肺癌单克隆抗体2F799F（ab′）2上

并测定抗体的修饰率、标记率、胶体含量、免疫活性、稳定性及安全性

探讨一种高效便捷的Tcm-2F799F（ab′）2放免显像药盒的研制方法。结果显示:已修饰2F7F（ab′）2的修饰效率（巯基/分子）为6.3

99Tcm的标记率为93.5%

99Tcm胶体含量为2.5%

修饰抗体有良好的稳定性和免疫活性;进行无菌、无热原、急慢性毒性实验

结果表明各项指标符合中国药典规定

所以建立的高效、便捷的单抗片断放免显像方法可以适应药盒化发展方向

该修饰、标记技术将对单抗F（ab′）2的放免显像的应用起到良好的作用。

Abstract

The aim of this work is to provide an accurate and scientific method of radionuclide imaging for small cell lung cancer with Tcm-2F7 F (99ab′ ) 2 . The monoclonal antibody 2F7 F (ab′ ) 2 segment was derived by iminothiolane and ligand exchange from Tcm O ( GH ) 99? 2 . Labeling efficiency was measured by paper chromatography. The label- ing efficiency of the labeled antibody 2F7 F ( ab′ ) 2 was 93.5%

with 2.5% colloidal Tcm in it. The competition test in 99 vitro showed that Tcm bound with the high affinity sites of antibody. The stability of the iminothiolane modified 99 antibody 2F7 F ( ab′ ) 2 could be kept at 4℃ for 3 months. In conclusions

the labelling method applied to the preparation of 99Tcm labeled antibody 2F7 F ( ab′ ) 2is successful

and appears to be equally applicable to other antibodies.

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