Study on trace elements distribution in rabbit metallothioneins   by synchrotron radiation X-ray fluorescence analysis

CHEN Chunying  1  ZHANG Peiqun  1  GAO Yuxi  1  CHAI Zhifang  1  HUANG Yuying  2  1

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Study on trace elements distribution in rabbit metallothioneins by synchrotron radiation X-ray fluorescence analysis

更新时间：2021-03-12

- Study on trace elements distribution in rabbit metallothioneins by synchrotron radiation X-ray fluorescence analysis
- NUCLEAR TECHNIQUES Issue 1, (2003)
- 作者机构：
  
  1. 中国科学院高能物理研究所核分析技术开放实验室
  2. 中国科学院高能物理研究所同步辐射国家实验室,北京,100039
- 作者简介：
- 基金信息：
  
  家自然科学基金 (2 0 0 710 3 2;1993 5 0 2 0 );中国科学院重大项目资助
- DOI：
  CLC： Q51
- Published：01 January 2003
- 稿件说明：
移动端阅览
[1]陈春英,章佩群,高愈希,柴之芳,黄宇营.用同步辐射X荧光分析法研究兔肝金属硫蛋白中的微量元素[J].核技术,2003(01):17-20.

CHEN Chunying 1 ZHANG Peiqun 1 GAO Yuxi 1 CHAI Zhifang 1 HUANG Yuying 2 1. Study on trace elements distribution in rabbit metallothioneins by synchrotron radiation X-ray fluorescence analysis[J]. Nuclear techniques, 2003, (1): 17-20.
[1]陈春英,章佩群,高愈希,柴之芳,黄宇营.用同步辐射X荧光分析法研究兔肝金属硫蛋白中的微量元素[J].核技术,2003(01):17-20. DOI：

CHEN Chunying 1 ZHANG Peiqun 1 GAO Yuxi 1 CHAI Zhifang 1 HUANG Yuying 2 1. Study on trace elements distribution in rabbit metallothioneins by synchrotron radiation X-ray fluorescence analysis[J]. Nuclear techniques, 2003, (1): 17-20. DOI：

摘要

采用同步辐射X荧光分析法（SRXRF）直接测定了兔肝金属硫蛋白（MT）中的金属离子含量

以及经过十二烷基硫酸钠 -聚丙烯酰胺凝胶电泳（SDS -PAGE）分离后凝胶板中蛋白条带上金属离子的分布。实验结果表明

兔肝MT - 1以含锌为主

占总金属含量的 97.9% ;兔肝MT - 2以含锌、镉为主。本工作表明

采用SRXRF技术可实现MT电泳分离后蛋白条带内金属离子分布的原位测定

Abstract

The distributions of metal ions in rabbit metallothionein (MT) and its protein band in sodium dodecyl sulfate-polyacrylamide gel after electrophoresis were determined by synchrotron radiation X-ray fluorescence (SRXRF) analysis. Results showed that MT-1 mostly contained zinc

which amounted to 97.9% of the total metallic content and MT-2 mainly contained zinc and cadmium. This paper also introduced the method to determine the relative amounts of protein-bound metal ions in situ by combination of electrophoretic separation and SRXRF analysis.

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Related Author

GAO Yuxi~1 CHEN Chunying~1 LI Bai~1 HUANG Yuying~2 HE Wei~2 DENG Guilong~3 LIU Yingbin~3 CHAI Zhifang~1 1

陈春英

李柏

黄宇营

何伟

邓贵龙

刘颖斌

柴之芳

Related Institution

Key Laboratory of Nuclear Analytical Techniques, Institute of High Energy Physics, the Chinese Academy of Sciences, Beijing 100049) 2

浙江大学第二附属医院普外科

中国科学院高能物理研究所核分析技术重点实验室

Institute of Modern Physics, the Chinese Academy of Sciences

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