Determination of trace elements in plasma protein by SRXRF

LIU Nianqing1  LIU Pingsheng1  HUANG Yuying1  HE Wei1  CHEN Defu2  ZHAO Jinyuan2 1

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Determination of trace elements in plasma protein by SRXRF

更新时间：2021-03-12

- Determination of trace elements in plasma protein by SRXRF
- NUCLEAR TECHNIQUES Issue 5, (2003)
- 作者机构：
  
  1. 中国科学院高能物理研究所
  2. 北京大学第三附属医院,北京,100039
  3. ,北京,100083
- 作者简介：
- 基金信息：
  
  国家自然科学基金(19975055,19935020和10175077) 资助
- DOI：
  CLC： R817
- Published：01 May 2003
- 稿件说明：
移动端阅览
[1]刘年庆,刘平生,黄宇营,何伟,陈德福,赵金垣.用同步辐射X荧光分析血浆蛋白质的微量元素[J].核技术,2003(05):329-332.

LIU Nianqing1 LIU Pingsheng1 HUANG Yuying1 HE Wei1 CHEN Defu2 ZHAO Jinyuan2 1. Determination of trace elements in plasma protein by SRXRF[J]. Nuclear techniques, 2003, (5): 329-332.
[1]刘年庆,刘平生,黄宇营,何伟,陈德福,赵金垣.用同步辐射X荧光分析血浆蛋白质的微量元素[J].核技术,2003(05):329-332. DOI：

LIU Nianqing1 LIU Pingsheng1 HUANG Yuying1 HE Wei1 CHEN Defu2 ZHAO Jinyuan2 1. Determination of trace elements in plasma protein by SRXRF[J]. Nuclear techniques, 2003, (5): 329-332. DOI：

摘要

用同步辐射X荧光（SRXRF）分析技术与凝胶色谱生化分离相结合

分析了用抗肿瘤药物顺铂处理过的和未处理过的小鼠血浆的大分子蛋白质。对以轻元素为基体的小量样品

用X荧光谱中的康普顿散射强度作为质量的因子

获得了在大于22kD分子量的蛋白质中Pt、S、Ca、Fe、Ni、Cu、Zn、Se、Br和Sr等元素含量在不同样品间的相对变化。由此推测

经顺铂处理后

Pt元素可与血浆中的大分子蛋白结合

含Cu和S的大分子蛋白含量明显升高

而含Zn的减少。

Abstract

An analytical method for the relative concentration of trace elements in plasma protein by gel chromatog-raphy combined with SRXRF (Synchrotron Radiation X-ray Fluorescence) was developed. The relative concentration of trace elements was obtained using the normalized Compton scattering intensity of protein in X-ray spectra as a function of protein mass. The male Kunming mice were treated with and without cisplatin. The relative change of elements (Pt

Br and Sr) contents in the fraction of the plasma proteins (>22kD) of the mice were obtained. The determination prove that the element Pt in plasma is bound with macro-molecular protein

and that Cu and S are increased while Zn is decrease in the fraction of the protein in mice treated with cisplatin.

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