A preparative technique of solid phase avidin and its  primary identification

SONG Shiping; TANG Guozhong

doi:null

您当前的位置：

首页 >

文章列表页 >

A preparative technique of solid phase avidin and its primary identification

更新时间：2021-03-12

- A preparative technique of solid phase avidin and its primary identification
- NUCLEAR TECHNIQUES Issue 5, (1999)
- 作者机构：
  
  中国科学院上海原子核研究所,上海,201800
- 作者简介：
- 基金信息：
- DOI：
  CLC： R392.12
- Published：01 May 1999
- 稿件说明：
移动端阅览
[1]宋世平,唐国忠.固相亲和素制备技术及初步鉴定[J].核技术,1999(05):305-308.

SONG Shiping, TANG Guozhong. A preparative technique of solid phase avidin and its primary identification[J]. Nuclear techniques, 1999, (5): 305-308.
[1]宋世平,唐国忠.固相亲和素制备技术及初步鉴定[J].核技术,1999(05):305-308. DOI：

SONG Shiping, TANG Guozhong. A preparative technique of solid phase avidin and its primary identification[J]. Nuclear techniques, 1999, (5): 305-308. DOI：

摘要

将亲和素（Ａｖ）与牛血清白蛋白（ＢＳＡ）通过偶合试剂连接制成联接物，利用活化后ＢＳＡ的改性使联接物一步包被到疏水固相上形成更加易于定量的固相亲和素。实验鉴定证明，这种固相亲和素具有良好的生物活性，初步应用效果良好，可推广应用于生物素－亲和素系统的免疫分析。

Abstract

A conjugate is formed of avidin(Av) and bovine serum albumin(BSA) through coupling reagents and this conjugate is bound to surface of support. Then

a sensitive

specific

competitive or noncompetitive immunoassay can be performed on the support. The experiments proved that this solid-phase avidin has good stability in RIA and can be used for automation of immunoassay.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Preparation of magnetic nanoparticles and its application in radioimmunoassay

Changes of serum leptin and c-peptide level in children with type 1 diabetic mellitus

Measurement of serum interleukin-6 and tumor necrosis factor-alpha in patients with Graves’ ophthalmopathy

Determination of EGF concentration in cancer tissues of colorectal cancer patients

Changes of bone density and serum osteocalcin levels in mid and late preganacy

Related Author

LI Guiping WANG Yongxian REN Fei ZHANG Hui

汪勇先

任非

张辉

齐绍康

DU Tongxin WANG Zizheng SUN Junjiang WANG Shukui QI Shaokang

王自正

孙君江

Related Institution

Shanghai Institute of Applied Physics, the Chinese Academy of Sciences

第一军医大学南方医院核医学科

第一军医大学南方医院核医学科上海 201800 第一军医大学南方医院核医学科

Nanjing First Hospital Affiliated to Nanjing Medical University

AI问答

Address：2019 Jialuo Road, Jiading, Shanghai, China Postal code：201800
Tel：021-39194977 Email：LHB@sinap.ac.cn
Technical support is provided by Beijing Founder electronics co., LTD 沪ICP备05005479号-1 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.
Total Visits：880886 Visits Today：413

⁰