The study on labelling conditions and in vitro stability of <sup>186</sup>Re-labelled anti-human brain glioma monoclonal antibody SZ-39

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The study on labelling conditions and in vitro stability of ¹⁸⁶Re-labelled anti-human brain glioma monoclonal antibody SZ-39

更新时间：2021-03-12

- The study on labelling conditions and in vitro stability of ¹⁸⁶Re-labelled anti-human brain glioma monoclonal antibody SZ-39
- NUCLEAR TECHNIQUES Issue 11, (1999)
- 作者机构：
  
  中国科学院上海原子核研究所!上海２０１８００
- 作者简介：
- 基金信息：
- DOI：
  CLC： R818.03
- Published：01 November 1999
- 稿件说明：
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[1]俎建华,吴元芳,董墨,李惠源,张雨龙.~(186)Re对抗人脑胶质瘤单克隆抗体的标记条件及其体外稳定性研究[J].核技术,1999(11):700-704.

The study on labelling conditions and in vitro stability of ¹⁸⁶Re-labelled anti-human brain glioma monoclonal antibody SZ-39[J]. Nuclear techniques, 1999, (11): 700-704.
[1]俎建华,吴元芳,董墨,李惠源,张雨龙.~(186)Re对抗人脑胶质瘤单克隆抗体的标记条件及其体外稳定性研究[J].核技术,1999(11):700-704. DOI：

The study on labelling conditions and in vitro stability of ¹⁸⁶Re-labelled anti-human brain glioma monoclonal antibody SZ-39[J]. Nuclear techniques, 1999, (11): 700-704. DOI：

摘要

用１８６Ｒｅ对抗人脑胶质瘤单克隆抗体（ＭＡｂＳＺ３９）进行了标记。研究了影响标记率的多种因素：如不同转移螯合剂、不同反应时间、ｐＨ及不同的ＳＺ３９还原剂等，最终找到了最佳反应条件，选用低ｐＨ的葡萄糖酸钠－盐酸缓冲溶液，标记率高达９６．２％。通过测量相同反应条件下标记率，发现１８６Ｒｅ比活度太低明显降低标记率。标记物进行了高效液相分离与纯化，并对纯化物作了体外稳定性测试，测定了不同条件下放化纯度随放置时间的变化关系。

Abstract

Monoclonal Antibody can be directly labelled with 186Re or 188Re. Usinganti-human brain glioma monoclonal antibody (SZ-39) as a model

we studied somefactors that influence labelling efficiency

such as PH of reaction mixture

varioustranschelation agents (sodium tartrate

sodium citrate

sodium gluconate)

thelabelling time and reduction agents (AA

DTT

2-ME) for reducing disulfide groupsin SZ39. At last

we established a better labelling method. The optimum PH wasshowed to be 2.54

sodium gluconate was used as transchelation agent. The labellingefficiency as determined by thin-layer chromatography and HPLC analysis was96.2%. The labelled SZ39 was tested by quantitative HPLC to determine theradiochemical purity and subsequent purification was collected for testing stability invitro. The labelled SZ39 has high radiochemical purity and was stable in 4°C by whenBSA was added.

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The study on labelling conditions and in vitro stability of 186Re-labelled anti-human brain glioma monoclonal antibody SZ-39

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The study on labelling conditions and in vitro stability of ¹⁸⁶Re-labelled anti-human brain glioma monoclonal antibody SZ-39