Crystallization and preliminary X-ray crystallographic analysis of the arsenic(Ⅲ) S-adenosylmethionine methyltransferase from rhodopseudanonas palustris

HUANG Linfei1; 2 YU Feng1 XU Chunyan1 TANG Lin1 HE Jianhua1 1

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Crystallization and preliminary X-ray crystallographic analysis of the arsenic(Ⅲ) S-adenosylmethionine methyltransferase from rhodopseudanonas palustris

更新时间：2021-03-05

- Crystallization and preliminary X-ray crystallographic analysis of the arsenic(Ⅲ) S-adenosylmethionine methyltransferase from rhodopseudanonas palustris
- NUCLEAR TECHNIQUES Vol. 35, Issue 5, (2012)
- 作者机构：
  
  1. 中国科学院上海应用物理研究所
  2. 中国科学院研究生院
- 作者简介：
- 基金信息：
  
  国家自然科学基金项目(31100528、31100529)资助
- DOI：
  CLC： X172
- Published：01 May 2012
- 稿件说明：
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[1]黄麟飞,郁峰,徐春艳,唐琳,何建华.沼泽红假单胞菌砷(Ⅲ)S-腺苷甲基转移酶的初步晶体学分析[J].核技术,2012,35(05):326-330.

HUANG Linfei1, 2 YU Feng1 XU Chunyan1 TANG Lin1 HE Jianhua1 1. Crystallization and preliminary X-ray crystallographic analysis of the arsenic(Ⅲ) S-adenosylmethionine methyltransferase from rhodopseudanonas palustris[J]. Nuclear techniques, 2012, 35(5): 326-330.
[1]黄麟飞,郁峰,徐春艳,唐琳,何建华.沼泽红假单胞菌砷(Ⅲ)S-腺苷甲基转移酶的初步晶体学分析[J].核技术,2012,35(05):326-330. DOI：

HUANG Linfei1, 2 YU Feng1 XU Chunyan1 TANG Lin1 HE Jianhua1 1. Crystallization and preliminary X-ray crystallographic analysis of the arsenic(Ⅲ) S-adenosylmethionine methyltransferase from rhodopseudanonas palustris[J]. Nuclear techniques, 2012, 35(5): 326-330. DOI：

摘要

含砷化合物有高毒性

一直是全球性的公共卫生难题。许多微生物能利用S-腺苷甲基转移酶（ArsM酶）催化有剧毒的无机As（Ⅲ）发生甲基化而解毒。为揭示酶促反应的真实机制

我们将来源于沼泽红假单胞菌（Rhodopseudanonas palustris）的ArsM酶（RpArsM）重组表达、纯化及结晶。在上海光源BL17U1线站收集蛋白晶体衍射数据

初步晶体学分析表明

RpArsM酶的晶体属于P212121空间群

晶胞参数分别是a=45.92

b=66.58

c=147.82

α=β=γ=90°

衍射分辨率达2.0。

Abstract

For the ubiquitous distribution and high toxicity in the ecosystem

arsenic contaminants have been a world-wide public health problem for decades.The enzyme ArsM has the ability of methylating the inorganic arsenite.To study mechanism of the process

the recombinant ArsM from Rhodopseudanonas palustris(RpArsM) was expressed

purified and crystallized.The RpArsM crystals were examined by X-ray crystallography on Beamline BL17U1 at Shanghai Synchrotron Radiation Facility

and the diffraction data were processed to a resolution of 2.0.The results show that the RpArsM crystals are of the P212121space group

with unit-cell parameters a=45.92

b=66.58

c=147.82 and α=β=γ=90°.

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