Effects of Fe<sub>3</sub>O<sub>4</sub> nanoparticles on secondary structure of cytochrome c by synchrotron radiation circular dichroism spectroscopy

WEI Huifang 1; 2 WANG Bing 2 GAO Di 1; 2 GUAN Ming 1 CHEN Hanqing 2 JIANG Jing 2 QU Xiuyun 2 ZHENG Lingna 2 OUYANG Hong 2 FENG Weiyue 2 1

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Effects of Fe₃O₄ nanoparticles on secondary structure of cytochrome c by synchrotron radiation circular dichroism spectroscopy

更新时间：2021-03-05

- Effects of Fe₃O₄ nanoparticles on secondary structure of cytochrome c by synchrotron radiation circular dichroism spectroscopy
- NUCLEAR TECHNIQUES Vol. 35, Issue 7, (2012)
- 作者机构：
  
  1. 吉林大学药学院
  2. 中国科学院高能物理研究所核分析技术院重点实验室及纳米生物效应与安全性院重点实验室
- 作者简介：
- 基金信息：
  
  国家自然科学基金(10905064,10975148)资助
- DOI：
  CLC： TB383.1
- Published：01 July 2012
- 稿件说明：
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[1]魏慧芳,汪冰,高迪,关铭,陈汉清,蒋静,屈秀云,郑令娜,欧阳宏,丰伟悦.同步辐射圆二色谱法研究Fe_3O_4纳米颗粒对细胞色素C结构的影响[J].核技术,2012,35(07):491-494.

WEI Huifang 1, 2 WANG Bing 2 GAO Di 1, 2 GUAN Ming 1 CHEN Hanqing 2 JIANG Jing 2 QU Xiuyun 2 ZHENG Lingna 2 OUYANG Hong 2 FENG Weiyue 2 1. Effects of Fe₃O₄ nanoparticles on secondary structure of cytochrome c by synchrotron radiation circular dichroism spectroscopy[J]. Nuclear techniques, 2012, 35(7): 491-494.
[1]魏慧芳,汪冰,高迪,关铭,陈汉清,蒋静,屈秀云,郑令娜,欧阳宏,丰伟悦.同步辐射圆二色谱法研究Fe_3O_4纳米颗粒对细胞色素C结构的影响[J].核技术,2012,35(07):491-494. DOI：

WEI Huifang 1, 2 WANG Bing 2 GAO Di 1, 2 GUAN Ming 1 CHEN Hanqing 2 JIANG Jing 2 QU Xiuyun 2 ZHENG Lingna 2 OUYANG Hong 2 FENG Weiyue 2 1. Effects of Fe₃O₄ nanoparticles on secondary structure of cytochrome c by synchrotron radiation circular dichroism spectroscopy[J]. Nuclear techniques, 2012, 35(7): 491-494. DOI：

摘要

四氧化三铁纳米颗粒（Fe3O4-NPs）在生物医学领域有着广泛的应用。通过同步辐射真空紫外圆二色谱（SRCD）和紫外可见（UV-Vis）吸收谱技术研究了Fe3O4纳米颗粒的粒径（10 nm和40 nm）对细胞色素C（Cyt c）结构的影响。结果表明

随着Fe3O4-NPs颗粒物浓度的增大

Cyt c在408 nm处的吸光度值逐渐下降。SRCD结果显示Cyt c与Fe3O4-NPs作用后

蛋白的α螺旋含量减少

β折叠含量增加;Fe3O4-NPs与Cyt C的作用强度具有尺寸依赖性

小尺寸的Fe3O4-NPs作用更为明显。

Abstract

upermagnetic iron oxide nanoparticles(Fe3O4-NPs) can promote many attractive functions in biomedical fields.In this study

the interaction of Fe3O4NPs(10 nm and 40 nm) with cytochrome c(Cyt c) was studied by synchrotron radiation circular dichroism(SRCD) and UV-Vis spectroscopy.After adding Fe3O4NPs in the Cyt c solution

the intensity of the Soret band(408 nm) decreased significantly.The SRCD characterization showed the loss of the α-helix structure and the occurrence of cytochrome c unfolding after Fe3O4-NPs treatment

meanwhile

the 10 nm Fe3O4-NPs had more prominent effects than the 40 nm ones.The results showed significant size-depended and dose-depended effects.This study provides important insight into the interaction of Fe3O4NPs with cytochrome c

which may be a useful guideline for further use of Fe3O4NPs.

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Effects of Fe3O4 nanoparticles on secondary structure of cytochrome c by synchrotron radiation circular dichroism spectroscopy

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摘要

Abstract

关键词

Keywords

references

Effects of Fe₃O₄ nanoparticles on secondary structure of cytochrome c by synchrotron radiation circular dichroism spectroscopy