18F-Pen-peptide was synthesized automatically in a PET-MF-IT-1 module using "click chemistry".18F-fluoropentyne was synthesized in reactor No.1 and distilled into reactor No.2

which was pre-loaded with peptide-N3

sodium ascorbate

and CuSO4/TBTA.After reacting at room temperature

the reaction mixture was purified by semi-HPLC

and the fine product 18F-Pen-peptide was collected by solid extraction.The cell uptake of 18F-Pen-peptide in A549 lung adenocarcinoma cells

which were or were not pre-treated with a chemical drug

was determined.The results showed that the yield of 18F-fluoropentyne was 51% from 18F-fluoride(EOS).The yield of 18F-Pen-peptide was nearly 99% from 18F-fluoropentyne.The total synthesis yield was(21.0?4.5)%(n=6

EOS).The radiochemistry purity was over 99%

and the specific activity was 870 GBq/μmol

respectively.It took 70 min for 18F-Pen-peptide starting from 18F-fluoride.After chemotherapy

the A549 cells uptake of 18F-Pen-peptide were(0.86±0.04)%

and the cell apoptosis rate was(10.23±2.43)%.The control A549 cells were(0.06±0.01)%

and the cells apoptosis rate was(1.02±0.31)%.The novel radiotracer 18F-Pen-peptide is a promising candidate for future apoptosis imaging in clinical settings.