A preliminary study of spinal cord microvasculature using synchrotron radiation imaging

WU Tianding 1 HU Jianzhong 1 ZENG Lei 1 PENG Guanyun3 LU Hongbin2 1

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A preliminary study of spinal cord microvasculature using synchrotron radiation imaging

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- A preliminary study of spinal cord microvasculature using synchrotron radiation imaging
- NUCLEAR TECHNIQUES Vol. 34, Issue 10, (2011)
- 作者机构：
  
  1. 中南大学湘雅医院脊柱外科
  2. 中国科学院上海应用物理研究所
  3. 中南大学湘雅医院运动医学科运动医学研究中心
- 作者简介：
- 基金信息：
  
  国家自然科学基金(No.81171698);湖南省自然科学基金(No.09JJ3047);湖南省卫生厅科研基金(No.B2007037)资助
- DOI：
  CLC： R730.44
- Published：01 October 2011
- 稿件说明：
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[1]吴天定,胡建中,曾磊,彭冠云,吕红斌.同步辐射成像应用于大鼠脊髓微循环研究的方法学初探[J].核技术,2011,34(10):726-730.

WU Tianding 1 HU Jianzhong 1 ZENG Lei 1 PENG Guanyun3 LU Hongbin2 1. A preliminary study of spinal cord microvasculature using synchrotron radiation imaging[J]. Nuclear techniques, 2011, 34(10): 726-730.
[1]吴天定,胡建中,曾磊,彭冠云,吕红斌.同步辐射成像应用于大鼠脊髓微循环研究的方法学初探[J].核技术,2011,34(10):726-730. DOI：

WU Tianding 1 HU Jianzhong 1 ZENG Lei 1 PENG Guanyun3 LU Hongbin2 1. A preliminary study of spinal cord microvasculature using synchrotron radiation imaging[J]. Nuclear techniques, 2011, 34(10): 726-730. DOI：

摘要

探讨了同步辐射显微断层成像技术（SRμCT）在大鼠脊髓微血管网络研究中的应用前景。正常SD大鼠3只

利用血管造影剂硅酮橡胶经升主动脉行血管造影术

次日取出下胸段脊髓

梯度酒精脱水后

冬绿油保存。标本先利用动物X射线机拍摄普通X片

然后在上海光源X射线成像与生物医学应用光束线站进行扫描和成像

并与原位厚组织切片的光镜摄片进行比较分析。SRμCT能对常规X射线成像无法观察到的大鼠脊髓微循环形态进行有效的成像和图形重构

清晰呈现出大鼠脊髓髓内微血管网络

可分辨的灰质内毛细血管直径最细至7.4μm左右。凭借其超高分辨率

SRμCT在血管相关性脊髓疾病的研究中具有广阔应用前景。

Abstract

Three male SD rats were subjected to angiography via ascending aorta with silicone rubber for investigating rat spinal cord microvasculature images by using synchrotron radiation-based micro-computed tomography.Low thoracic cord was harvested

followed by X-ray radiography

synchrotron radiation micro-imaging and thick histological section in situ.The microvascular images were clearly projected by synchrotron radiation.The rendered 3D images produced more details of the microvascular arrangement in rat spinal cord harmonized with histology.It is of great potential to investigate microvessel-related spinal cord disease with synchrotron radiation-based micro-computed tomography.

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