Effect of dose and DNA concentration on DNA damage induced by protons

SUI Li1; 2 WANG Xiao1 ZHOU Pingkun2 NI Meinan1 KONG Fuquan1 YANG Lei1 LIU Jiancheng1 ZHAO Kui1 1

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Effect of dose and DNA concentration on DNA damage induced by protons

更新时间：2021-03-05

- Effect of dose and DNA concentration on DNA damage induced by protons
- NUCLEAR TECHNIQUES Vol. 34, Issue 6, (2011)
- 作者机构：
  
  1. 中国原子能科学研究院
  2. 军事医学科学院放射与辐射医学研究所
- 作者简介：
- 基金信息：
  
  国家自然科学青年基金(10805078);国家杰出青年科学基金(30825011)资助
- DOI：
  CLC： Q691
- Published：01 June 2011
- 稿件说明：
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[1]隋丽,王潇,周平坤,倪嵋楠,孔福全,杨磊,刘建成,赵葵.质子剂量和DNA浓度对DNA损伤的影响[J].核技术,2011,34(06):447-451.

SUI Li1, 2 WANG Xiao1 ZHOU Pingkun2 NI Meinan1 KONG Fuquan1 YANG Lei1 LIU Jiancheng1 ZHAO Kui1 1. Effect of dose and DNA concentration on DNA damage induced by protons[J]. Nuclear techniques, 2011, 34(6): 447-451.
[1]隋丽,王潇,周平坤,倪嵋楠,孔福全,杨磊,刘建成,赵葵.质子剂量和DNA浓度对DNA损伤的影响[J].核技术,2011,34(06):447-451. DOI：

SUI Li1, 2 WANG Xiao1 ZHOU Pingkun2 NI Meinan1 KONG Fuquan1 YANG Lei1 LIU Jiancheng1 ZHAO Kui1 1. Effect of dose and DNA concentration on DNA damage induced by protons[J]. Nuclear techniques, 2011, 34(6): 447-451. DOI：

摘要

利用HI-13串列加速器产生的16 MeV质子

使用凝胶电泳技术对添加和未添加甘露醇的pUC19质粒DNA溶液中DNA分子形态变化进行了观测

研究了同一剂量下不同DNA浓度及同一DNA浓度下不同剂量对DNA链断裂产额的影响。结果表明

未添加甘露醇溶液中DNA浓度效应较明显。两种体系下DNA均表现出明显的剂量响应

即分子形态由完整的超螺旋形态逐渐向发生单链断裂的开环形态或双链断裂的线性形态变化

且未添加甘露醇的体系链断裂产额相对更高

表明甘露醇可清除自由基

有效降低质子致DNA单链和双链断裂的发生。此外

还研究了10 ng/μL和100 ng/μL DNA浓度下

不同剂量对DNA损伤的影响。结果显示

DNA溶液浓度越低

达到同样损伤所需的辐照剂量越小。

Abstract

The pUC19 plasmid DNA solutions in different DNA concentrations

with or without mannitol

were irradiated by 16 MeV protons to different doses.Changes in the DNA form were observed by means of gel electrophoresis in order to study effects of dose and DNA concentration on yields of DNA strand breaks.The results indicate that the dose and DNA concentration response exist in two solution systems

the forms of DNA changed from intact supercoiled to open circular

in a linear relationship with the dose.The yields of DNA strand breaks in DNA solution without mannitol are much more than that those with mannitol

illuminating that mannitol can scavenge the free radicals and reduce the yields of DNA single and double strand breaks induced by protons efficiently.In addition

the dose effects of DNA damage were investigated at a concentration of 10 or 100 ng/?L.The results show that dilute DNA solution need lower dose than the dense solution in observing similar degree of damage.

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赵葵

蔡明辉

隋丽

倪嵋楠

3 1

2 ZHAO Kui1

2 KONG Fuquan1 ZHAN Yong2 WANG Xiao1 NI Meinan1 SUI Li1 CAI Minghui1

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Related Institution

河北工业大学理学院天津

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China Institute of Atomic Energy,Beijing 102413,China) 2

Xinjiang Institute of Physics, Academia Sinica

Fudan University

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