Effect of low energy N~+ ion beam implantation on the secondary structure and activity of α-amylase

XU Qiang1 YANG Tiqiang2; 3 WANG Miao4 1

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Effect of low energy N~+ ion beam implantation on the secondary structure and activity of α-amylase

更新时间：2021-03-05

- Effect of low energy N~+ ion beam implantation on the secondary structure and activity of α-amylase
- NUCLEAR TECHNIQUES Vol. 34, Issue 2, (2011)
- 作者机构：
  
  1. 宝鸡文理学院物理与信息技术系
  2. 内蒙古自治区离子束生物工程重点实验室
  3. 内蒙古大学物理科学与技术学院
  4. 宝鸡文理学院图书馆
- 作者简介：
- 基金信息：
  
  陕西省科学技术研究计划(2010K01-078)项目;陕西省教育厅自然科学专项基金(08JK214);宝鸡文理学院重点项目(ZK1015)
- DOI：
  CLC： O657.39
- Published：01 February 2011
- 稿件说明：
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[1]许强,杨体强,王苗.低能N~+注入对α-淀粉酶二级结构及其活性的影响[J].核技术,2011,34(02):147-150.

XU Qiang1 YANG Tiqiang2, 3 WANG Miao4 1. Effect of low energy N~+ ion beam implantation on the secondary structure and activity of α-amylase[J]. Nuclear techniques, 2011, 34(2): 147-150.
[1]许强,杨体强,王苗.低能N~+注入对α-淀粉酶二级结构及其活性的影响[J].核技术,2011,34(02):147-150. DOI：

XU Qiang1 YANG Tiqiang2, 3 WANG Miao4 1. Effect of low energy N~+ ion beam implantation on the secondary structure and activity of α-amylase[J]. Nuclear techniques, 2011, 34(2): 147-150. DOI：

摘要

本文介绍利用圆二色（CD）光谱法研究20 keV N+注入对α-淀粉酶二级结构的影响

测定其CD光谱

分析了酶活性变化的原因。结果表明:在1015–1016/cm2范围

不同注量的低能N+注入对α-淀粉酶的α-螺旋、β-折叠、β-转角及无规卷曲相对含量的影响程度不同。低能N+注入使α-淀粉酶活性发生改变

酶活性的改变与其空间结构的变化密不可分。

Abstract

amples of the α-amylase were treated with 1015–1016 ions/cm2 of 20 keV N+.The secondary structure of the ion-implanted α-amylase was studied by circular dichroism

and activity change of the α-amylase was analyzed.The results showed that in the dose range under investigation

the N+ implantation affected the relative contents of α-helix

β-sheet

β-turn and random coil of the α-amylase

but no significant differences were found between the samples implanted to different doses.The activity of the N+-implanted α-amylase changed

with a relationship between the activity increase and the conformation change.

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Related Institution

Key Laboratory of Heavy Ion Physics, Ministry of Education, Peking University, Beijing 100871) 2

北京大学化学与分子工程学院应用化学系

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郑州大学离子束生物工程省重点实验室郑州450052,合肥230031,郑州450052

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