Effects of nanoparticles on stability of cell DNA studied by SRCD

ZHANG Mingyi1 WANG Peng2 XING Gengmei1 ZHANG Jun2 1

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Effects of nanoparticles on stability of cell DNA studied by SRCD

更新时间：2021-03-05

- Effects of nanoparticles on stability of cell DNA studied by SRCD
- NUCLEAR TECHNIQUES Vol. 34, Issue 2, (2011)
- 作者机构：
  
  1. 中国科学院高能物理研究所
  2. 北京理工大学生命学院
- 作者简介：
- 基金信息：
  
  国家自然科学基金(10875135);科技部国家重点基础研究发展计划项目(2009CB930204)资助
- DOI：
  CLC： TB383.1
- Published：01 February 2011
- 稿件说明：
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[1]张铭倚,王鹏,邢更妹,张钧.同步辐射圆二色谱分析纳米颗粒对细胞DNA稳定性的影响[J].核技术,2011,34(02):134-138.

ZHANG Mingyi1 WANG Peng2 XING Gengmei1 ZHANG Jun2 1. Effects of nanoparticles on stability of cell DNA studied by SRCD[J]. Nuclear techniques, 2011, 34(2): 134-138.
[1]张铭倚,王鹏,邢更妹,张钧.同步辐射圆二色谱分析纳米颗粒对细胞DNA稳定性的影响[J].核技术,2011,34(02):134-138. DOI：

ZHANG Mingyi1 WANG Peng2 XING Gengmei1 ZHANG Jun2 1. Effects of nanoparticles on stability of cell DNA studied by SRCD[J]. Nuclear techniques, 2011, 34(2): 134-138. DOI：

摘要

选用30 nm和50 nm羧基化聚苯乙烯微球（COOH-PBs）作用于RAW264.7巨噬细胞

用真空紫外圆二色光谱（SRCD）分析细胞DNA二级结构的可能变化

并用彗星电泳验证此法的可行性和优越性。研究结果显示

两种颗粒都致使DNA螺旋度降低及单链程度增加

30 nm COOH-PBs造成的损伤甚于50 nm

表明纳米颗粒影响DNA的稳定性有粒径依赖性。SRCD为深入研究纳米颗粒对细胞DNA稳定性的影响及其可能原因提供了一个可行的研究方法和途径。

Abstract

To investigate influence of different-sized nanoparticles on stability of cell DNA

RAW264.7 strains were incubate with 30 nm and 50 nm COOH-labeled polystyrene beads(COOH-PBs).Synchrotron radiation circular dichroism spectroscopy(SRCD) was used to analyze possible transformation of the secondary structure of DNA

which was further confirmed by means of comet assay.The CD spectra indicated that both particles could reduce helicity of the DNA and increase the degree of DNA single strands.The 30 nm-PBs treatment did more damage than the 50 nm-PBs treatment.This suggests a size-dependent influence.It is demonstrated that SRCD provides a reliable method to investigate the stability of cell DNA influenced by nanoparticles.

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