Studies on watermelon somatic cell mutant of resistance to fusaric acid (FA) by low energy Ar~+ ion beam irradiation

WANG Haobo1; 2  GAO Xiuwu2  GU Yunhong1  CHENG Guowang1; 2  YU Zengliang1 1

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Studies on watermelon somatic cell mutant of resistance to fusaric acid (FA) by low energy Ar~+ ion beam irradiation

更新时间：2021-03-12

- Studies on watermelon somatic cell mutant of resistance to fusaric acid (FA) by low energy Ar~+ ion beam irradiation
- NUCLEAR TECHNIQUES Issue 8, (2003)
- 作者机构：
  
  1. 中国科学院等离子体物理研究所离子束生物工程学重点实验室,中国科学院等离子体物理研究所离子束生物工程学重点实验室,中国科学院等离子体物理研究所离子束生物工程学重点实验室,中国科学院等离子体物理研究所离子束生物工程学重点实验室,合肥,230031
  2. 安徽丰乐农业科学技术研究院合肥230031,合肥230031,合肥230031,合肥,230031
- 作者简介：
- 基金信息：
  
  国家自然科学基金(19890300-4)资助
- DOI：
  CLC： S651
- Published：01 August 2003
- 稿件说明：
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[1]王浩波,高秀武安徽丰乐农业科学技术研究院,谷运红,程国旺,余增亮.离子束诱变西瓜体细胞抗镰刀菌酸突变体研究[J].核技术,2003(08):609-612.

WANG Haobo1, 2 GAO Xiuwu2 GU Yunhong1 CHENG Guowang1, 2 YU Zengliang1 1. Studies on watermelon somatic cell mutant of resistance to fusaric acid (FA) by low energy Ar~+ ion beam irradiation[J]. Nuclear techniques, 2003, (8): 609-612.
[1]王浩波,高秀武安徽丰乐农业科学技术研究院,谷运红,程国旺,余增亮.离子束诱变西瓜体细胞抗镰刀菌酸突变体研究[J].核技术,2003(08):609-612. DOI：

WANG Haobo1, 2 GAO Xiuwu2 GU Yunhong1 CHENG Guowang1, 2 YU Zengliang1 1. Studies on watermelon somatic cell mutant of resistance to fusaric acid (FA) by low energy Ar~+ ion beam irradiation[J]. Nuclear techniques, 2003, (8): 609-612. DOI：

摘要

将通过能量为25keV、注量为6.24×1016/cm2的Ar+ 辐射处理后的三份西瓜种子接种在含有15mg / L镰刀菌酸（Fusaric acid

FA）的MS（Murashige

Skoog）培养基上获得无菌苗

以其子叶为外植体接种在MS+2mg/L 6-苄氨基嘌呤（Benzylaminopruine

BA）+15mg/L FA培养基上进行诱导生芽

将抗性再生芽转接到MS+0.2mg/L萘乙酸（Naphthylacetic acid

NAA）+15mg/L FA培养基上

进行抗FA的再生苗的生根诱导培养。结果表明

离子束辐射处理和FA对感病西瓜种子3-27和YH-5的发芽率及成苗都有显著抑制

两者的复合抑制作用更强;离子束辐射处理过的两个感病西瓜种子抗FA的芽诱导再生率和生根诱导率都高于对应的不经过离子束辐射处理的对照材料

提高幅度在材料之间、芽再生和根再生之间有差异。

Abstract

Three kinds of watermelon seeds irradiated by Ar+ ion beam (25keV

6.24×1016 ions/cm2) were inoculated in MS medium with 15mg/L FA. Cotyledons from the sterile seedling as explants were inoculated in MS+BA 2.0mg/L+FA 15mg/L. And the adventitious shoots of resistance to FA were cultured in MS+NAA 0.2mg/L+FA 15mg/L. The results showed that both the irradiation of Ar+ and FA affected the germination rate and seedling of watermelon line 3-27 and YH-5

and the joint effect of Ar+ and FA showed an enhanced restraint. The adventitious shoot and rootage induction rate from the seeds irradiated by Ar+ were respectively bigger than the unirradiated seeds in 3-27 and YH-5. The increasing ranges were different between two watermelon lines and between the shoot and rootage induction rates.

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