The effect of estrogen antanogist ICI-182780 on the regulatory action of ZDYIOI on M<sub>2</sub> receptor density in CHOm<sub>2</sub> cells

WANG Xuesong HUYaer XIAZongqin

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The effect of estrogen antanogist ICI-182780 on the regulatory action of ZDYIOI on M₂ receptor density in CHOm₂ cells

更新时间：2021-03-12

- The effect of estrogen antanogist ICI-182780 on the regulatory action of ZDYIOI on M₂ receptor density in CHOm₂ cells
- NUCLEAR TECHNIQUES Issue 11, (2003)
- 作者机构：
  
  1. 上海第二医科大学细胞调控研究室
  2. 上海第二医科大学细胞调控研究室,上海,200025
- 作者简介：
- 基金信息：
  
  国家自然科学基金(30070926)资助
- DOI：
  CLC： R96
- Published：01 November 2003
- 稿件说明：
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[1]王雪松,胡雅儿,夏宗勤.雌激素拮抗剂ICI-182780对ZDY101调节CHOm_2细胞M_2受体密度的影响[J].核技术,2003(11):845-848.

WANG Xuesong HUYaer XIAZongqin. The effect of estrogen antanogist ICI-182780 on the regulatory action of ZDYIOI on M₂ receptor density in CHOm₂ cells[J]. Nuclear techniques, 2003, (11): 845-848.
[1]王雪松,胡雅儿,夏宗勤.雌激素拮抗剂ICI-182780对ZDY101调节CHOm_2细胞M_2受体密度的影响[J].核技术,2003(11):845-848. DOI：

WANG Xuesong HUYaer XIAZongqin. The effect of estrogen antanogist ICI-182780 on the regulatory action of ZDYIOI on M₂ receptor density in CHOm₂ cells[J]. Nuclear techniques, 2003, (11): 845-848. DOI：

摘要

CHOm2细胞加雌二醇、ZDY101或等体积溶媒DMSO培养后通过3H-NMS结合反应测定M受体密度

并与同时加雌激素受体拮抗剂ICI-182780的细胞作对比。结果显示:雌二醇组M受体密度为（123.7±23.9）fmol·mg-1

加ICI-182780后为（84.8±19.8）fmol·mg-1;相应的DMSO对照组为（89.6±22.9）fmol·mg-1

加ICI后为（66.9±22.5）fmol·mg-1;雌二醇组加ICI-182780后M受体密度降低的幅度远大于DMSO对照组。ZDY101组M受体密度为（147.3±36.9）fmol·mg-1

加ICI-182780后为（120.8±40.4）fmol·mg-1;相应的DMSO对照组为（95.9±24.0）fmol·mg-1

加ICI-182780后为（77.0±22.3）fmol·mg-1。表明雌激素和ZDY101都具有的提高M受体密度的功效

雌激素的药效可被ICI抑制

而ZDY101的药效不被ICI抑制

说明ZDY101有不同于雌激素的作用机制。

Abstract

After the treatment of estradiol

ZDYIOI or DMSO (solvent) to cultured CHOm2 cells for a certain peirod

M-receptor density was determined by 3H-NMS binding assay. Parallel observations were carried out with or without the addition of ICI-182780. The Results show that the density of M receptor in cells treated with estradiol or DMSO control were 123.7±23.9 and 89.6±22.9fmol/mg protein

and these values dropped down to 84.8±19.8 and 66.9±22.5fmol/mg with the presence of ICI-182780. The density of M receptor in cells treated by ZDYIOI or DMSO control were 147.3±36.9 and 95.9±24.0fmol/mg

and dropped to 120.8±40.4 and 77.0±22.3fmol/mg protein with the presence of ICI-182780. ICI-182780 caused a much greater decrease of M receptor density in estradiol treated cells than DMSO control cells indicating that estradioi increased the M receptors through action on estradiol receptor. The magnitude of decrease of M receptor density induced by ICI-182780 in ZDYIOI treated group was similar to that in DMSO controls

indicating that the increase of M receptor density induced by ZDY101 could not be inhibited by ICI-182780 and hence had a mechanism different from estradiol.

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The effect of estrogen antanogist ICI-182780 on the regulatory action of ZDYIOI on M2 receptor density in CHOm2 cells

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摘要

Abstract

关键词

Keywords

references

The effect of estrogen antanogist ICI-182780 on the regulatory action of ZDYIOI on M₂ receptor density in CHOm₂ cells