Studies on kinetic properties for binding of [<sup>125</sup>I]-<italic style="font-style: italic">α</italic>-bungarotoxin to nicotinic ACh receptors from chick

Shiming Chen

您当前的位置：

首页 >

文章列表页 >

Studies on kinetic properties for binding of [¹²⁵I]-α-bungarotoxin to nicotinic ACh receptors from chick

更新时间：2022-09-13

- Studies on kinetic properties for binding of [¹²⁵I]-α-bungarotoxin to nicotinic ACh receptors from chick
- Nuclear Techniques Vol. 10, Issue 9, (1987)
- 作者机构：
  
  军事医学科学院药理毒理研究所
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Received：05 July 1985，
  
  Published：1987-09
- 稿件说明：
移动端阅览
Shiming Chen. Studies on kinetic properties for binding of [¹²⁵I]-α-bungarotoxin to nicotinic ACh receptors from chick[J]. Nuclear techniques, 1987, 10(9): 53-56,44,65.
DOI：

Shiming Chen. Studies on kinetic properties for binding of [¹²⁵I]-α-bungarotoxin to nicotinic ACh receptors from chick[J]. Nuclear techniques, 1987, 10(9): 53-56,44,65. DOI：

摘要

从鸡脑及骨骼肌中分离提取烟碱样胆碱能受体，用[

125

I]-

-银环蛇毒素进行受体-配体结合动力学研究。结果表明，这种结合呈二级动力学反应性质。脑视叶中提纯的受体和骨骼肌中分离的受体，

值分别为129pM及4pM（25℃）。烟碱、箭毒碱等可抑制受体－配体的结合反应并测得各自的抑制常数及Hill系数。

Abstract

N-AChRs of chicken brain (either from optic lobe or from the rest of brain) and skeletal muscle were extracted in presence of protease inhibitors. The receptor of brain was further purified to homogeneity (pI=5.4) by affinity chromatography. The kinetic properties for binding of [

125

I]-

-BTX to detergent-solubilized and purified receptors from optie lobe and detergent-solubilized receptor from skeletal muscle were studied. It was confirmed that they are nicotinic. The binding of

-BTX to AChR from optic lobe was consistent with a simple bimolecular reaction. In contrast

it was biphasic to the muscle AChR. Dissociation constants (K

) for AChR-[

125

I]-

-BTX complex from optic lobe and from muscle were 129 pM and 4 pM respectively at 25℃. The reaction of [

125

I]-α-BXT with AChR was inhibited by agonists or antagonists and the

and h values were determined by Hill plot analysis of the data.

关键词

Keywords

references

陈世铭，国外医学分子生物学分册 , 6 , 2 , 56 ( 1984 )。

Z. Vogel et al. , Proc. Nat. Acad. Sci. USA. , 69 , 3180 ( 1972 ).

J. O. Dolly et al. , Biochemistry , 16 , 5053 ( 1977 ).

M. M. S. Lo et al. , Eur. J. Biochem. , 116 , 155 ( 1981 ).

U. Quast et al. , Biochemistry , 17 , 2405 ( 1978 ).

T. Heidmann et al. , Eur. J. Biochem. , 94 , 255 ( 1979 ).

B. J. Morley ， Pharmac. Ther. , 15 , 111 ( 1981 ).

G-K. Wang et al. , J. Biol. Chem. , 253 , 8507 ( 1978 ).

U. Z. Littauer et al. , Neurotransmitters and their receptors , John Wiley and Sons Ltd. , New york , 1980 , p. 271 .

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

No data

Related Author

No data

Related Institution

No data

AI问答

Address：2019 Jialuo Road, Jiading, Shanghai, China Postal code：201800
Tel：021-39194977 Email：LHB@sinap.ac.cn
Technical support is provided by Beijing Founder electronics co., LTD 沪ICP备05005479号-1 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.
Total Visits：880565 Visits Today：92

⁰

Studies on kinetic properties for binding of [125I]-α-bungarotoxin to nicotinic ACh receptors from chick

DOI：

摘要

Abstract

关键词

Keywords

references

Studies on kinetic properties for binding of [¹²⁵I]-α-bungarotoxin to nicotinic ACh receptors from chick