125-iodine-labelled second antibody separation radioimmunoassay for the cyclic GMP

Jun Fang; Bin Gong; Qizhong Mo; Xuping Qian; Jumei Wang; Xingwei Kuang; Rong Chen; Baihui Wu

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125-iodine-labelled second antibody separation radioimmunoassay for the cyclic GMP

更新时间：2022-09-13

- 125-iodine-labelled second antibody separation radioimmunoassay for the cyclic GMP
- Nuclear Techniques Vol. 09, Issue 10, (1986)
- 作者机构：
  
  1.上海中医学院
  2.上海试剂研究所
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Received：28 October 1983，
  
  Published：1986-10
- 稿件说明：
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Jun Fang, Bin Gong, Qizhong Mo, et al. 125-iodine-labelled second antibody separation radioimmunoassay for the cyclic GMP[J]. Nuclear techniques, 1986, 09(10): 54-56,32,F3.
DOI：

Jun Fang, Bin Gong, Qizhong Mo, et al. 125-iodine-labelled second antibody separation radioimmunoassay for the cyclic GMP[J]. Nuclear techniques, 1986, 09(10): 54-56,32,F3. DOI：

摘要

环磷酸鸟苷（cGMP）和环磷酸腺苷组成一对矛盾。它们对细胞的生长、繁殖及生理过程起着重要的调节作用，在中医药基础理论及临床研究中日益受到重视。但是cGMP在体液及组织中含量甚微，因此需要有一个灵敏、简易、特异的测定方法。

Abstract

This paper describes iodine labelled radioimmunoassay for the cyclic GMP. Preparations of antigen

antibody and radioligand are described. A second antibody separation of simple high sensitivity method for the assay of cyclic GMP is presented.

The cross-reactivity of this cyclid GMP antiserum with cyclic AMP and GTP are 0.008% and 3.1×10

-3

% respectively.

Sensitivity of the method is 1.5 fmol cyclic GMP per tube. Recovery of the method is 104.0±4.7% and variety coefficient in batch is 2.8%.

Cyclic GMP content in the plasma of normal man is 4.60±0.70 pmol/ml.

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references

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H. Cailla et al . , Anal. Biochem. , 48 , 62 （ 1972 ）.

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E. K. Framdsen et al . , Life Sci. , 19 , 529 （ 1976 ）.

贺师鹏等，生物化学与生物物理进展 , 6 , 60 （ 1983 ）。

陈南春，中华核医学杂志， 4 ， 2 ， 87 （ 1984 ）。

R. M. C. Dawson et al . , Data for biochemical research ., 1969 , p. 625 .

丁秀娟，竞争放射分析，原子能出版社， 1981 年，第 225 页。

戴滕昌等，核技术 , 3 , 27 （ 1981 ）。

华家柽等，实用蛋白质化学技术，上海科学技术出版社， 1982 年， 411 页。

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