Quantitative detection of bacteria by radiorespirometric technique

Qi Chen; Limin Zhang; Guangcai He; Jie Sun; Zekun Fu; Zhengbin Zuo

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Quantitative detection of bacteria by radiorespirometric technique

更新时间：2022-09-13

- Quantitative detection of bacteria by radiorespirometric technique
- Nuclear Techniques Vol. 08, Issue 3, (1985)
- 作者机构：
  
  1.第二军医大学
  2.海军医学研究所
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Published：1985
- 稿件说明：
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Qi Chen, Limin Zhang, Guangcai He, et al. Quantitative detection of bacteria by radiorespirometric technique[J]. Nuclear techniques, 1985, 08(3): 27-29,33,72,73.
DOI：

Qi Chen, Limin Zhang, Guangcai He, et al. Quantitative detection of bacteria by radiorespirometric technique[J]. Nuclear techniques, 1985, 08(3): 27-29,33,72,73. DOI：

摘要

细菌放射呼吸测量法是利用放射核素监测细菌生长、代谢的技术。也是一种专用于探测微生物代谢释放

的技术。因为二氧化碳是许多重要生物反应的天然产物，所以通过对CO

的测定可以了解很多生物现象。尤其是细菌的体外培养几乎在代谢过程中都能放出CO

，因此培养基中加入

C-标记的营养物（如

C-葡萄糖、

C-氨基酸等），经细菌的代谢降解作用可生成

气体，通过仪器测量放射性，可定量地检测细菌的数量。该类技术具有快速、敏感和客观等特点，用途较多。本研究试图在我们已建立的液体闪烁法细菌放射测定的基础上

[1]

，观察需氧菌的培养和定量测定、水和食品中细菌总数的检测以及尿样品中细菌总数的检查等，以提供细菌放射测量法实际应用的参考。

Abstract

This paper describes a radiorespirometric method for the quantitative detection ofbacteria. By using an alternative modified method and liquid scintillation counting system 15 species of aerobic bacteria and specimens of water

cooked food and urine were tested. The

released in batch cultures of bacteria was measured continuously and cumulatively. Detection time of growth index ≥3 was used to measure number of bacteria positive for the presence of bacterial growth. The results indicated that this technique could offer a rapid

sensitive and dependable approach to quantitative microbiology.

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references

陈杞等，核技术， 3 ， 9 （ 1983 ）。

E.U. Buddemeyer et al ., J. Nucl. Med ., 19 , 619 （ 1978 ）.

中华人民共和国卫生部，生活饮用水卫生标准TJ20-76（试行本）， 1973 年，第 3 页。

中华人民共和国卫生部：食品卫生标准GB 2726-81 , 1982 年，第 25 页。

U. Bachrach and Z. Bachrach , Appl. Microbiol. , 28 , 160 （ 1974 ）.

B. J. Stewart et al ., Applied and Environmental Microbiology , 40 , 223 （ 1980 ）.

H. G. Frank et al ., Evaluation of a one hour radiometric screening test for urine culture . （Abst.） Ann, Meet , ASM . 1978 , 301 （ C146 ）.

I. Leader and R. Potts , Rapid diagnsis of urinary tract infections by BACTEC and gram stain . 3rd International Symposium on rapid method and automation in microbiology （Abst）. 1981 , p. 12 .

L. S. Gall and G. Johnson , Radiometric methods for rapid detection of bacteriuria . ASM 73rd Annual Meeting , 1973 , p. 99 .

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