The preparation of <sup>125</sup>I-DNA

Deyuan Lu; Jinying Wang; Zhongxian Ye

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The preparation of ¹²⁵I-DNA

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- The preparation of ¹²⁵I-DNA
- Nuclear Techniques Vol. 06, Issue 3, (1983)
- 作者机构：
  
  中国人民解放军海军医学研究所
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Received：09 July 1982，
  
  Published：1983-06
- 稿件说明：
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Deyuan Lu, Jinying Wang, Zhongxian Ye. The preparation of ¹²⁵I-DNA[J]. Nuclear techniques, 1983, 06(3): 51-52,78.
DOI：

Deyuan Lu, Jinying Wang, Zhongxian Ye. The preparation of ¹²⁵I-DNA[J]. Nuclear techniques, 1983, 06(3): 51-52,78. DOI：

摘要

125

I-DNA是个重要的标记化合物，在生物学、免疫学、病理学和某些疾病的临床诊断、愈后观察等方面都具有十分重要的意义。因此，寻找简便的方法制备

125

I-DNA是急需解决的问题。最初，人们用一氯化碘法制备

125

I-DNA，需把DNA转化成溶于有机溶剂的盐，从而导至DNA降解，影响了产品质量；继后采用N-碘丁二酰亚胺法制备

125

I-DNA，但

125

I-DNA的比放射性很低。近期，人们采用生物标记法

［1］

即

125

P－脱氧尿嘧啶核苷（

125

I-UdR）由大肠杆菌转化生成

125

I-DNA。用这种方法制得的

125

I-DNA具有较好的生物活性和纯度，其缺点是操作十分繁杂，

125

I的利用率低，

125

I-DNA的比放射性也很低。另外，还用三氯化铊法制备

125

I-DNA

［1

2］

，虽然方法简便，

125

I-DNA的比放射性也较高，但是三氯化铊不稳定，必须新鲜配制，使用十分不便。本文采用三氧化二铊制备

125

I-DNA，克服了上述缺陷。

125

I利用率达30~60%，

125

I-DNA的比放射性达10µCi/µg，放射性杂质小于1%。方法十分简便，利于推广使用。

Abstract

125

I-DNA is a labelled compound of significance to biology

immunology

pathology clinic diagnosis and the study of therapeutic effects. The methods of preparatiag

125

I-DNA by iodination with iodine chloride

N-iodosuccinimide

thallium trichloride and incorporating

125

I-UdR into DNA in E. coli cells are not satisfactory.

125

I-DNA described in the paper was prepared by a method using thallium oxide. It had a labelling rate of 30~60% with

125

I-DNA specific radioactivity about 10µCi/µg

and of good purity (Radioactive impurity being less than 1%). This method is simple and practical.

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references

Shalom A. Leom et al . , US 3897212 , 1975 .

S. L. Commerford , Biochemistry , 10 , 11 , 1993 ( 1971 ).

李振甲等，放射免疫分析手册，科学出版社， 1980年8月．

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The preparation of 125I-DNA

DOI：

摘要

Abstract

关键词

Keywords

references

The preparation of ¹²⁵I-DNA