The modified metabolism-scintillation system and experimental conditions for radiometric detection of bacterial growth

Qi Chen; Limin Zhang; Guangcai He; Jie Sun; Zekun Fu; Zhengbin Zuo; Ping Wang

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The modified metabolism-scintillation system and experimental conditions for radiometric detection of bacterial growth

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- The modified metabolism-scintillation system and experimental conditions for radiometric detection of bacterial growth
- Nuclear Techniques Vol. 06, Issue 3, (1983)
- 作者机构：
  
  1.中国人民解放军第二军医大学
  2.中国人民解放军海军医学研究所
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Received：13 November 1892，
  
  Published：1983-06
- 稿件说明：
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Qi Chen, Limin Zhang, Guangcai He, et al. The modified metabolism-scintillation system and experimental conditions for radiometric detection of bacterial growth[J]. Nuclear techniques, 1983, 06(3): 9-13,74,75.
DOI：

Qi Chen, Limin Zhang, Guangcai He, et al. The modified metabolism-scintillation system and experimental conditions for radiometric detection of bacterial growth[J]. Nuclear techniques, 1983, 06(3): 9-13,74,75. DOI：

摘要

为了使液体闪烁法用于细菌放射测量的研究，本文提出一种 "顶端闪烁管-代谢瓶"装置和

捕集-闪烁系统，可使测量效率达60%以上，培基装量增至8~10ml，初步进行了肠炎沙门氏菌、金黄色葡萄球菌、大肠杆菌和腊样杆菌的放射测量试验，当接种菌量在10

~10

/ml时，培养2~4小时可得到阳性结果。对放射测量法的某些影响因素（如试验装置、细菌种类及接种浓度等）进行了观察与分析。推荐用液体闪烁法测量的生长指数大于3做为评定细菌生长阳性的指标。

Abstract

In order to use the liquid scintillation counter for the bacterial radiometric assay

a modified metabolism-scintillation vial was designed and a new scintillation trap system was tried. With this assembly good counting effeciency was obtained (

60%) and larger amounts of medium (8~10ml per vial) was held. Generally

it can be detected after 2~4 hour incubation with inoculum (salm. enterilidis

E. coli

Staphy. aureus

B. cereus) of 10

~10

/ml. In radiometric measurement of bacteria some influencing factors (e. g. type of metabolism-scintillation vial

concentration of bacterial inoculum etc) were investigated. The growth index exceeding 3 with liquid scintillation technique could be considered as positive reading of bacterial growth.

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references

F. H. Deland & H. N. Wagner Jr. , J. Lab. Clin. Med ., 75 , 529 ( 1970 ).

E. V. Buddemeyer , Appl. Microbiol ., 28 , 177 , ( 1974 ).

E. V. Buddemeyer et al . , Clin. Chem ., 22 , 1459 ( 1976 ).

E. V. Buddemeyer et al . , J. Nucl. Med ., 19 , 619 ( 1978 ).

B. G. Yanfco et al . , 3rd intern, sym. rapid. Method and automation in microbiol ., 1981 , P. 17 .

N. Tran & H. N. Wagner , J. Nucl. Med ., 19 , 61 ( 1978 ).

M. M. Rehani , J. Nucl. Med ., 20 , 1090 ( 1979 ).

R. G. Ganatra et al . , J. Nucl. Med ., 21 , 480 ( 1980 ).

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