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上海第二医科大学
Received:16 April 1987,
Published:1988-06
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Guoping Feng, Ningyu Yi, Zongqin Xia. A study of the experimental conditions in beta-adrenergic receptor binding assay[J]. Nuclear techniques, 1988, 11(6): 61-64.
本文对
β
-肾上腺素受体放射配基分析法中的缓冲液、膜受体制备条件、膜蛋白用量、结合与游离配基的分离方法及非特异性结合的控制等方面作了探讨和改进,使方法的稳定性和可靠性有了明显的提高,并对所建方法进行了较完整的考核。
The experimental conditions in binding assay of beta-adrenergic receptor with
3
H-dihydroalprenolol (
3
H-DHA) were studied. The buffer medium
the membrane preparation
the influence of protein quantity
the separation of bound/free radioligand and the source of nonspecific binding were investigated. An improved procedure was given and its specificity and stability proved quite satisfactory.
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