A new Lowry's technique for quantitative measurement of protein

Gc Chen; Wenquan Zou; Jianzhong Sun; Yanggang Zhang; Bohua Shu; Shenpei Liu; Xiaoliang Gong

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A new Lowry's technique for quantitative measurement of protein

更新时间：2022-08-16

- A new Lowry's technique for quantitative measurement of protein
- Nuclear Techniques Vol. 13, Issue 12, (1990)
- 作者机构：
  
  同济医科大学
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Received：17 July 1989，
  
  Published：01 December 1990
- 稿件说明：
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Gc Chen, Wenquan Zou, Jianzhong Sun, et al. A new Lowry's technique for quantitative measurement of protein[J]. Nuclear techniques, 1990, 13(12): 756-759.
DOI：

Gc Chen, Wenquan Zou, Jianzhong Sun, et al. A new Lowry's technique for quantitative measurement of protein[J]. Nuclear techniques, 1990, 13(12): 756-759. DOI：

摘要

液体闪烁计数中的淬灭效应被应用于Lowry氏蛋白质定量测定。结果表明：不同浓度的显色蛋白样品与仪器计数效率之间存在较好的线性关系。实验证明液闪测定法较传统的分光光度计测定法具有误差小、可测范围大、便于自动化分析等特点。

Abstract

According to the quenching principle in beta ray measurement

liquid scintillation counters were used for quantitative measurement of protein. The results show linear relationship between the colored protein samples with different concentrations and the counting rate of LSC. It has been proved that LSC method is less erroneous and has larger measurement range than the traditional photoelectric colorimetry

and the analysis is easy to be automatized.

关键词

Keywords

references

O. H. Lowry et al . , J. Biol. Chem . , 193 （ 1951 ）, 265 .

上海化工学院分析化学教研组编，分析化学，下册，第一版，人民教育出版社出版，上海， 1978 年，第29页。

H. B. William , Nuclear medicine , Second Edition , McGiaw-Hill Book Company , New York. 1971 , p. 97 .

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