A new technique of ion beam tritium labelling

Nianbao Zhang; Shugang Sheng; Fuzeng Yao; Binghua Xie; Meiying Zhou; Rongzhen Cao; Einwei Yu; Desong Zhong; Ruifang Qin; Guoping Wang

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A new technique of ion beam tritium labelling

更新时间：2022-08-16

- A new technique of ion beam tritium labelling
- Nuclear Techniques Vol. 13, Issue 10, (1990)
- 作者机构：
  
  中国科学院上海原子核研究所
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Received：04 December 1989，
  
  Published：01 October 1990
- 稿件说明：
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Nianbao Zhang, Shugang Sheng, Fuzeng Yao, et al. A new technique of ion beam tritium labelling[J]. Nuclear techniques, 1990, 13(10): 599-606.
DOI：

Nianbao Zhang, Shugang Sheng, Fuzeng Yao, et al. A new technique of ion beam tritium labelling[J]. Nuclear techniques, 1990, 13(10): 599-606. DOI：

摘要

本文介绍一种先进的氚离子束标记技术，用于蛋白质、肽和非挥发性有机化合物的氚标记。在该技术中，加速受激发的氚离子束，去轰击固体样品，发生氢－氚交换反应。氚化大豆胰蛋白酶抑制剂、核糖核酸酶A、弹性蛋白和茯苓聚糖等十一种化合物已用此法制备。氚化粗产物经透析、离子交换色层和凝胶过滤等方法纯化后，蛋白质和聚糖的比活性一般可达37GBq/mmol以上，没有放射性副产物和具有与天然物质相同的生物活性。氚化蛋白质的氨基酸分析表明，在蛋白质中，His、 Tyr和Phe残基具有较高的比活性而Val、Ile和Ser残基的比活性较低。

Abstract

In this paper a new technique is reported for tritium labelling of proteins

peptides and other nonvolatile organic compounds. A tritium ion beam is accelerated to bombard solid sample target for producing tritium exchange with hydrogen. The tritium labelling method has been applied to tritiated soybean trypsin inhibitor

ribonuclease A

elastin

pachyman and others totalled 11. After purifying by dialysis

ion exchange chromatography and gel filtration

the tritiated proteins and polysaccharide were obtained with specific activity over 37GBq/mmol

without decomposition and with biological activity well preserved. By amino acid analysis of tritiated protein it was shown that the relative specific radioactivities for His.

Tyr. and Phe. residues were higher while those for Val.

Ile. and Ser. residues were lower.

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references

E. A. Evans , Tritium and its compounds , 2nd Ed. , Butterworths , London , 1974 .

J. A. Elvidge and J. R. Jones , Isotopes: Essential chemistry and application , London the chemical society London WIA OBN , 1980 , p. 400 .

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C. VonHolt et al . , Biochim. Biophys. Acta , 38 （ 1960 ）, 88 .

M. Noyer et al . , J. Labelled Compd. Radiopharm . , 12 （ 1976 ）, 365 .

F. H. White et al . , Anal. Biochem . , 30 （ 1969 ）, 295 .

R. Ehrenkaufer et al . , J. Labelled Compd. Radiopharm . , 14 （ 1978 ）, 271 .

G. A. Bush et al . , J. Biol. Chem . , 256 （ 1981 ）, 12213 .

B. C. Richardson et al . , J. Labelled Compd. Radiopharm . , 21 （ 1984 ）, 914 .

W. Kisiel et al . , Biochemistry , 15 （ 1976 ）, 4901 .

H. Umezawa , Enzyme inhibitors of microbial orgin , University Park Press , Tokyo , 1972 .

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