Studies of the radioiodination methods for anti-CEA monoclonal antibody

Liyi Lu; Zhangshu Han; Daihong Dai; Han Lin; Yi Tian

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Studies of the radioiodination methods for anti-CEA monoclonal antibody

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- Studies of the radioiodination methods for anti-CEA monoclonal antibody
- Nuclear Techniques Vol. 12, Issue 11, (1989)
- 作者机构：
  
  1.北京师范大学
  2.中国医学科学院放射医学研究所
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Received：23 November 1987，
  
  Published：1989-11
- 稿件说明：
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Liyi Lu, Zhangshu Han, Daihong Dai, et al. Studies of the radioiodination methods for anti-CEA monoclonal antibody[J]. Nuclear techniques, 1989, 12(11): 648-652.
DOI：

Liyi Lu, Zhangshu Han, Daihong Dai, et al. Studies of the radioiodination methods for anti-CEA monoclonal antibody[J]. Nuclear techniques, 1989, 12(11): 648-652. DOI：

摘要

本文报道用几种碘标记方法（Iodogen、ChT、NBS、Bolton-Hunter试剂等）制备了

125

I（131I）-抗CEA单克隆抗体。产品通过Sephadex G-50（或-75）柱凝胶过滤纯化后，经纸层析和高压液相层析法鉴定，其放化纯度达到使用要求；用免疫放射双抗体夹心法测定，标记抗体能保持良好的免疫活性。同时我们对上述几种碘标方法进行比较研究，结果NBS法标记率最高，达98%

Iodogen法免疫活性保持最好、与CEA的净结合数达9960cpm（3ng CEA/250µl）。

Abstract

The preparation of

125

I (

131

I) -anti-CEA monoclonal antibody by using several radioiodination methods (Iodogen

ChT

NBS

and Bolton-Hunter reagent etc.) was described. Purification of the product was carried out by the Sephadex G-50 (or 75) gel-filtration column. Its radiochemical purity checked by paper chromatography and HPLC was satisfactory for use. The labelled antibody showed good immunoreactivity

when it was measured by IRMA-bi-antibody sandwich method. The comparative studies for above several radioiodination methods were carried out

among which the iodination efficiency of NBS method was the best (98%) while the immunoreactivity of Iodogen method was the best and specific binding of labelled antibody to CEA was 9960 cpm (3ng CEA/250 µl) .

关键词

Keywords

references

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William C. Eckelman et al ., Cancer Research , 40 （ 1980 ）, 3036 .

John M. Ferens et al ., J. Nucl. Med. , 25 （ 1984 ）, 367 .

Gary S. David et al ., Biochem. , 13 ., （ 1974 ）, 1014 .

L. C. Knight et al ., J. Label. Comp. Radiophar. , 18 （ 1981 ）, 50 .

A. E. Bolton and W. M. Hunter , Biochem. J. , 133 （ 1973 ）, 529 .

T. W. Frederck et al ., Anal. Biochem. , 69 （ 1975 ）, 339 .

J. F. Pamela et al ., Biochem. Biophys. Res. Commun. , 80 （ 1978 ）, 849 .

Olli Vuolteenaho et al ., Life Sciences , 27 （ 1980 ）, 27 .

P. Reay , Ann. Clin. Biochem. , 19 （ 1982 ）, 129 .

林　汉等，中华核医学杂志， 6 （ 1986 ）， 3 : 137 .

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A comparative study of the Iodogen etc. methods of radioiodination for anti CEA monoclonal antibody

Related Author

Ming Cheng

Related Institution

Institute of Biological-Preparation

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