Labelling of human red cell superoxide dismutase with <sup>125</sup>I by the conjugation method

Meiyu Qiang; Limin Zhang; Jie Sun; Delin Wu; Qi Chen

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Labelling of human red cell superoxide dismutase with ¹²⁵I by the conjugation method

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- Labelling of human red cell superoxide dismutase with ¹²⁵I by the conjugation method
- Nuclear Techniques Vol. 12, Issue 5, (1989)
- 作者机构：
  
  第二军医大学
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Received：25 November (1987，
  
  Published：1989-05
- 稿件说明：
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Meiyu Qiang, Limin Zhang, Jie Sun, et al. Labelling of human red cell superoxide dismutase with ¹²⁵I by the conjugation method[J]. Nuclear techniques, 1989, 12(5): 304-308.
DOI：

Meiyu Qiang, Limin Zhang, Jie Sun, et al. Labelling of human red cell superoxide dismutase with ¹²⁵I by the conjugation method[J]. Nuclear techniques, 1989, 12(5): 304-308. DOI：

摘要

本文介绍一种新的碘化标记高比放射性人SOD的方法。结果表明，

125

I-SOD标记产品放射化学纯度大于96%，比活性达0.74—1.15MBq/µg。放射免疫测定表明，该标记物有较高的放射免疫性，当抗血清稀释度为1:10000时，

125

I-SOD特异性结合抗体，其结合率达56%。

Abstract

Human superoxide dismutase (SOD) was labelled with high specific radioactivity by conjugating to an

125

I-iodinizated acylating agent. Radiochemical purity of

125

I-SOD was more than 96% and its specific activity was 0.74—1.15 MBq/µg. Radioimmunoassay showed that the tracer had a higher radioimmunoactivity. The

125

I-SOD antigen was able to combine with its SOD antibody specifically. The binding rate was 56% at the antiserum dilution of 1:10000.

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references

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张丽民等，核技术， 9 （ 1987 ）, 6 : 46 。

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Villano BCD et al . , J. Immunol. Methods , 29 （ 1979 ）, 253 .

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B. J. Morris , Clin. Chim. Acta , 73 （ 1976 ）, 213 .

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Related Author

徐懿群

何荣霞

俞惠新

李卫一

曹国宪

Limin Zhang

Jie Sun

Delin Wu

Related Institution

Jiangsu Institute of Nuclear Medicine

State Key Laboratory of Nuclear Medicine,Jiangsu Institue of Nuclear Medicine

核医学国家重点实验室

江苏省原子医学研究所核医学国家重点实验室

The Second Millitary Medical College

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Labelling of human red cell superoxide dismutase with 125I by the conjugation method

DOI：

摘要

Abstract

关键词

Keywords

references

Labelling of human red cell superoxide dismutase with ¹²⁵I by the conjugation method