Determination of deoxyadenosine 5'-<italic style="font-style: italic">α</italic>-thiotriphosphate by <sup>31</sup>P nuclear magnetic resonance spectroscopy

Dongzhu Zheng; Keming Zhan

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Determination of deoxyadenosine 5'-α-thiotriphosphate by ³¹P nuclear magnetic resonance spectroscopy

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- Determination of deoxyadenosine 5'-α-thiotriphosphate by ³¹P nuclear magnetic resonance spectroscopy
- Nuclear Techniques Vol. 12, Issue 4, (1989)
- 作者机构：
  
  中国科学院上海原子核研究所
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Received：21 October 1987，
  
  Published：1989-04
- 稿件说明：
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Dongzhu Zheng, Keming Zhan. Determination of deoxyadenosine 5'-α-thiotriphosphate by ³¹P nuclear magnetic resonance spectroscopy[J]. Nuclear techniques, 1989, 12(4): 240-242.
DOI：

Dongzhu Zheng, Keming Zhan. Determination of deoxyadenosine 5'-α-thiotriphosphate by ³¹P nuclear magnetic resonance spectroscopy[J]. Nuclear techniques, 1989, 12(4): 240-242. DOI：

摘要

利用

P核磁共振技术，对脱氧腺苷-[

-硫代]三磷酸盐的构型进行了研究。在

P-NMR实验中观察到在δ43.1ppm处，有两个三重峰，根据其化学位移和偶合裂分提供的信息，确证了这化合物是5'-脱氧腺苷-[

-硫代]三磷酸盐。同时

P-NMR图谱表明，此化合物是以单一的SP或RP异构体存在。

Abstract

In the preparation of high specific activity deoxyadenosine 5'- [

-35S] thiotriphosphate

the structure of deoxyadenosine 5'-

-thiotriphosphate (5'-dATP

S) was determined by

P-NMR. In the proton coupled

P NMR spectrum

there was a triplet of doublets (J

=27Hz

=6Hz) at 43.1ppm. Therefore this compound was recognized to be 5'-isomer. The

P-NMR spectrum showed that it exists in either SP or RP configuration.

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references

Jin-Tann Chen , Nucleic Acids Research , 11 （ 1983 ）, 11 : 3737 .

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Determination of deoxyadenosine 5'-α-thiotriphosphate by 31P nuclear magnetic resonance spectroscopy

DOI：

摘要

Abstract

关键词

Keywords

references

Determination of deoxyadenosine 5'-α-thiotriphosphate by ³¹P nuclear magnetic resonance spectroscopy