1

H NMR

used for structure analysis of

2

H labelled compounds only by peak area reduction in

1

H NMR spectrum

is restricted in a non-coupling proton system with

2

H content more than 10% otherwise the results will be obscure. As an improvement

2

H NMR is directly used to observe the

2

H NMR signal and calculate percentage of

2

H in every exchange group from the area of peaks. Deuteriated compounds were prepared by acid-catalyzed exchange at 135℃ in dilute acid media (DC1-D

2

O) and using PtO

2

.

2

H NMR parameters are as follows: resonance frequency of 15.25 MHz

pulse width of 7.5µs

pulse delay time of 200 ms

and noise decoupling of 0.5 kHz.