Radioreceptor assay of human growth hormone

Sanjun Zheng; Xiangtong Lin; Wanting He

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Radioreceptor assay of human growth hormone

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- Radioreceptor assay of human growth hormone
- Nuclear Techniques Vol. 11, Issue 12, (1988)
- 作者机构：
  
  上海医科大学附属华山医院
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Received：02 November 1987，
  
  Published：1988-12
- 稿件说明：
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Sanjun Zheng, Xiangtong Lin, Wanting He. Radioreceptor assay of human growth hormone[J]. Nuclear techniques, 1988, 11(12): 46-50.
DOI：

Sanjun Zheng, Xiangtong Lin, Wanting He. Radioreceptor assay of human growth hormone[J]. Nuclear techniques, 1988, 11(12): 46-50. DOI：

摘要

以妊娠兔肝细胞膜碎片为受体制剂，用乳过氧化物酶法或Iodogen法碘化标记人生长激素，建立生长激素放射受体分析法。本方法灵敏度为0.67±0.11ng/ml。用RRA和RIA两种方法同时对72例正常成人、102例肢端肥大症患者测定血清GH浓度，结果表明正常成人两种方法测定结果无显著差异（

0.05），而肢端肥大症患者的RRA值

RIA值（术前

0.01，术后

0.05）。

Abstract

A radioreceptor assay for human growth hormone was developed. The receptor preparation was 25

000g pellet from the livers of pregnant rabbits. Iodination of GH with

125

I was preformed by the methods of Lactoperoxidase and Iodogen. The sensitivity of assay was 0.67±0.11 ng/ml serum.

Serum hGH levels in 72 cases of normal subjects

102 cases of acromegaly were mea sured by RRA

and the results were compared with those obtained by RIA.

关键词

Keywords

references

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上海实验生物研究所和上海第一医学院华山医院同位素室，生物化学与生物物理进展，（ 1975 ）， 1 ： 31 .

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N. Hizuka et al . , Endocrinol. Jpn. , 31 （ 1984 ）, 6 : 719

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