Synthesis of <sup>3</sup>H-NSP and tritium labelling of bio-macromolecules

xiongqi Gong; Zuoen Zhang; Zhimin Li; Manji Sun; Fengzhen Li; Han Zhang; Jiabei Wang

您当前的位置：

首页 >

文章列表页 >

Synthesis of ³H-NSP and tritium labelling of bio-macromolecules

更新时间：2022-08-16

- Synthesis of ³H-NSP and tritium labelling of bio-macromolecules
- Nuclear Techniques Vol. 11, Issue 1, (1988)
- 作者机构：
  
  军事医学科学院药理毒理研究所
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Published：1988-01
- 稿件说明：
移动端阅览
xiongqi Gong, Zuoen Zhang, Zhimin Li, et al. Synthesis of ³H-NSP and tritium labelling of bio-macromolecules[J]. Nuclear techniques, 1988, 11(1): 45.
DOI：

xiongqi Gong, Zuoen Zhang, Zhimin Li, et al. Synthesis of ³H-NSP and tritium labelling of bio-macromolecules[J]. Nuclear techniques, 1988, 11(1): 45. DOI：

摘要

在蛋白质及多肽等生物大分子研究中，往往需要用放射性同位素进行标记以达到追踪或检测的目的。1981年Dolly等报道用N-琥珀酰亚胺[2，3-

H]丙酸－酯（

H-NSP）标记银环蛇毒素成功，1983年Yuis等发表了合成

H-NSP的方法。我们参考此法，改进了反应溶剂和纯化方法，合成了

H-NSP，总产率17.3%，比度525.4GBq/mmol，放化纯＞98%，随后又将丙烯酸与N-羟基琥珀酰亚胺先缩合成琥珀酰亚胺丙烯酸酯，再氚化制备

N-NSP，获得成功，产率80%，比度1.33TBq/mmol，放化纯＞95%，从而使引入标记元素改在最后一步，缩小了合成量，节省了氚气，并提高了产率。用我们合成的

H-NSP分别标记了丙种球蛋白、乙酰胆碱酯酶、过氧化氢酶和眼镜蛇毒素都获成功，并保持生物活性。

Abstract

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Exploring the source of life energy --A research of synchrotron radiation on ATP synthase

Preparation of tritium labelled thymidine with high specific activity

Investigation on 3H-labelled bilirubin for Study of blood-brain barrier

Preparation of ￣3H -Adriamuycin and its monoclonal antibodies against human brain glioma

Labelling of potent narcotic analgesics by the low tritium pressure gas-liquid exchange method

Related Author

张新夷

TIAN Liang ZHANG Xinyi

曹蓉珍

Dong Mo Cao Rongzhen

曹蓉珍

董墨

张雨龙

周瑞菊

Related Institution

复旦大学同步辐射研究中心

Synchrotron Radiation Research Center, Fudan University

Shanghai Institute of Nuclear Research, the Chinese Academy of Sciences

Shanghai Institute of Nuclear Research, Academia Sinica

Shanghai Institute of Nuclear Research, Academia Sinica, shanghai 201800)Lan Qing

AI问答

Address：2019 Jialuo Road, Jiading, Shanghai, China Postal code：201800
Tel：021-39194977 Email：LHB@sinap.ac.cn
Technical support is provided by Beijing Founder electronics co., LTD 沪ICP备05005479号-1 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.
Total Visits：880565 Visits Today：92

⁰

Synthesis of 3H-NSP and tritium labelling of bio-macromolecules

DOI：

摘要

Abstract

关键词

Keywords

references

Synthesis of ³H-NSP and tritium labelling of bio-macromolecules