Radioiodinated labelling of superoxide dismutase

Limin Zhang; Jie Sun; Delin Wu; Meiyu Qiang; Qi Chen

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Radioiodinated labelling of superoxide dismutase

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- Radioiodinated labelling of superoxide dismutase
- Nuclear Techniques Vol. 10, Issue 6, (1987)
- 作者机构：
  
  第二军医大学
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Received：10 November 1985，
  
  Published：1987-06
- 稿件说明：
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Limin Zhang, Jie Sun, Delin Wu, et al. Radioiodinated labelling of superoxide dismutase[J]. Nuclear techniques, 1987, 10(6): 46-49,63.
DOI：

Limin Zhang, Jie Sun, Delin Wu, et al. Radioiodinated labelling of superoxide dismutase[J]. Nuclear techniques, 1987, 10(6): 46-49,63. DOI：

摘要

超氧物歧化酶（Superoxide dismutase简称SOD）是从红细胞、肝和其它哺乳动物组织分离而得的金属蛋白酶，主要存在于需氧环境的每一个器官中，分子量32000

[1]

，含有两个相同的亚单位，每一亚单位含铜和锌原子各一个

[2]

。

Abstract

Radioiodinated labelling of superoxide dismutase was performed using the chloramine-T method. The product was separated from the mixture by Sephadex G-75 column chromatography using 0.05 mol potassium phosphate buffer (pH7.8). Specific activity and radiochemical purity of the product were 166kBq/µg and more than 99% respectively. Labelled antigen (the product) bound with antibody (>50%).

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Keywords

references

J. M. Hartz et al. , J. Biol. Chem. , 274 , 7043 ( 1972 ).

E. M. David , Biochemistry , Academic Press Inc. , New York , 1977 , p. 624 .

J. M. McCord et al. , Proc. Nat. Acad. Sci., U. S. A ., 65 , 1024 ( 1971 ).

K. Kelly , Arch. Biochem. Biophys. , 190 , 531 ( 1978 ).

B. C. D. Villano et al. , Methods in Enzymology . Vol. 74 , Imm unochemical Techniques Part C . 1981 . p. 359 .

M. S. Howard , J. Biol. Chem. , 255 , 6758 ( 1980 ).

B. M. Joseph , Biochem. biophys. Acta , 45 , 382 ( 1960 ).

吴德林等，超氧物歧化酶的抗血清制备及其放射免疫测定，第二军医大学学报 , 7 , 3 , 215 ( 1986 )。

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Related Author

LIU Ning

JIN Jiannan

ZHANG Shuyuan

YANG Yuanyou

Meiyu Qiang

Limin Zhang

Jie Sun

Delin Wu

Related Institution

Institute of Nuclear Science and Technology, Sichuan University

教育部辐射物理及技术重点实验室

教育部辐射物

Second Military Medical College

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