Study on the xanthomonas oryzae <sup>14</sup>C tracer method

Shuyu Wang

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Study on the xanthomonas oryzae ¹⁴C tracer method

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- Study on the xanthomonas oryzae ¹⁴C tracer method
- Nuclear Techniques Vol. 10, Issue 2, (1987)
- 作者机构：
  
  东北农学院
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Received：21 May 1986，
  
  Published：1987-02
- 稿件说明：
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Shuyu Wang. Study on the xanthomonas oryzae ¹⁴C tracer method[J]. Nuclear techniques, 1987, 10(2): 52-53,F3.
DOI：

Shuyu Wang. Study on the xanthomonas oryzae ¹⁴C tracer method[J]. Nuclear techniques, 1987, 10(2): 52-53,F3. DOI：

摘要

水稻白叶枯病（

Xanthomonas oryzae

）是重要的细菌病害之一，十九世纪末发生于日本，六十年代起逐渐传播于亚洲各国，在中国原仅发生于南方稻区，现已传播北方各省。在大发生年份造成严重为害，要想弄清白叶枯细菌的侵染规律，必须采用先进的检测方法。目前，国内外用分离培养、噬菌体法

[1]

、镜检观察喷菌法

[2]

、浓缩接种法和反向间接血凝法

[1]

进行了大量的研究工作。这些方法对种子、稻株、杂草和稻田水等带菌的检验起了很大作用。但对检验病菌的侵染来源及植物体内微量细菌的潜伏存在有一定的困难。我们经过五年试验，初步探索出白叶枯细菌的

C标记、制样和测量方法。

Abstract

Compound beef extract agar medium with

C of specific radioactivity of 10µCi/ml to label xanthomonas oryzae bacterium by culture.Using sample concentrated filter paper-liquidscientillation method to determine the sample bacterium. By the method of x

tests

the stability the apparatus was determined. taking from 19 determinations of the back-ground and the sample

the 2σerror of the average value of 13 caounting data were duse to uncertain the existance of the tracer bacterium.

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references

许志刚等，植物病理学报， 10 ， 2 ， 65 （ 1980 ）。

伊阪実人，今月の农药， 8 ， 62 （ 1974 ）。

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Study on the xanthomonas oryzae 14C tracer method

DOI：

摘要

Abstract

关键词

Keywords

references

Study on the xanthomonas oryzae ¹⁴C tracer method