Preparation of <sup>14</sup>C-aminoacyl-tRNA

Fude Fang; Jin Zuo; Guanyun Wu

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Preparation of ¹⁴C-aminoacyl-tRNA

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- Preparation of ¹⁴C-aminoacyl-tRNA
- Nuclear Techniques Vol. 09, Issue 3, (1986)
- 作者机构：
  
  中国医学科学院基础医学研究所
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Received：08 June 1984，
  
  Published：1986-03
- 稿件说明：
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Fude Fang, Jin Zuo, Guanyun Wu. Preparation of ¹⁴C-aminoacyl-tRNA[J]. Nuclear techniques, 1986, 09(3): 44-45,60.
DOI：

Fude Fang, Jin Zuo, Guanyun Wu. Preparation of ¹⁴C-aminoacyl-tRNA[J]. Nuclear techniques, 1986, 09(3): 44-45,60. DOI：

摘要

在研究蛋白质生物合成的机制及蛋白质合成抑制剂作用机理等工作中，经常需要用

C-氨酰－tRNA作为标记底物进行示踪实验。

C-氨酰-tRNA无市售产品，一般都是各实验室自行制备。迄今为止，在国内文献中尚未见制备此类标记物的报道。我们结合有关的工作，以天然tRNA和L-

C-氨基酸作为底物，用酶促法制备了几种

C-氨酰－tRNA。

Abstract

Four kinds of

C-aminoacyl-tRNA were preparated by means of enzymatic method. Specific activity of products prepared was 7476—132394 cрm per mg tRNA. It has been found that these products possess good biological activity in vitro.

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references

P. Leder and H. Bursztyn , Proc. Nat. Acad. Sci. , USA , 56 , 1579 ( 1966 ).

K. Moldave , Methods in enzymology , VI. Academic Press , New York and London , 1963 , p. 757 .

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Preparation of 14C-aminoacyl-tRNA

DOI：

摘要

Abstract

关键词

Keywords

references

Preparation of ¹⁴C-aminoacyl-tRNA