The PIXE technique was used to analyse the elemental composition of human hair. The PIXE experimental facility used in the Institute of High Energy Physics was described. The methods of sample preparation and elemental quantitative analysis were introduced in detail. The accepted intercomparison material for trace element studies from human hair was analysed and the results were in good agreement with those by other well established traditional methods. The hair from patients with hepatolenticular degeneration(Wilson's disease) was also analysed and compared with that from normal persons. The apparent abnormality of calcium and copper contents in the patient's hair were not observed.