Labeling pectin and dextran with <sup>14</sup>C

Limin Zhang; Qi Chen; Jie Sun; Dihua Zhang

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Labeling pectin and dextran with ¹⁴C

更新时间：2022-08-16

- Labeling pectin and dextran with ¹⁴C
- Nuclear Techniques Vol. 06, Issue 6, (1983)
- 作者机构：
  
  第二军医大学
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Received：31 December 1982，
  
  Published：1983-12
- 稿件说明：
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Limin Zhang, Qi Chen, Jie Sun, et al. Labeling pectin and dextran with ¹⁴C[J]. Nuclear techniques, 1983, 06(6): 62-64,76.
DOI：

Limin Zhang, Qi Chen, Jie Sun, et al. Labeling pectin and dextran with ¹⁴C[J]. Nuclear techniques, 1983, 06(6): 62-64,76. DOI：

摘要

多糖的放射性标记物是进行示踪代谢和化学结构研究等重要的工具之一。迄今为止，用

的生物学方法或植物合成法较为复杂；而利用

C-氰化物与多糖起缩合反应，制得

C-标记的多糖，可获得满意的结果，也是目前较为实用的有效途径。

Abstract

Labelad pectin and dextran was obtained by condensation of the polysaccharides with

C-labeled cyanide in the presence of a carbonate-bicarbonate buffer. The products were purified using an ion exchange resin. Experimental results show that the structures of labeled polysaccharides were not change. Evidently specific activities of products were 3~4Ci/mg (

C-pectin) and 1μCi/mg (

C-dextran) . Radiochemical purities of products were more than 97%. The content of monosaccharide is less than 3%.

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references

HS Isbell and DC Washingtan, USP , 2653931 （ 1953 ）.

陈杞等，柚皮果胶代血浆的体内分布和代谢研究，待发表。

川端晶子等，营养て食粮， 29 ， 4 , 241 （ 1976 ）。

DAR. Simmons , Biochem. J ., 84 , 2 , 356 （ 1962 ）.

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Labeling pectin and dextran with 14C

DOI：

摘要

Abstract

关键词

Keywords

references

Labeling pectin and dextran with ¹⁴C