Enzymatic radioiodination of polypeptide hormones and their stability in storage

Xiangtong Lin; Shouzhen Deng; Weiren Zhang; Jinming Zhang

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Enzymatic radioiodination of polypeptide hormones and their stability in storage

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- Enzymatic radioiodination of polypeptide hormones and their stability in storage
- Nuclear Techniques Vol. 06, Issue 1, (1983)
- 作者机构：
  
  上海第一医学院核医学研究所华山医院临床核医学研究室
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Received：20 November 1981，
  
  Published：1983-02
- 稿件说明：
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Xiangtong Lin, Shouzhen Deng, Weiren Zhang, et al. Enzymatic radioiodination of polypeptide hormones and their stability in storage[J]. Nuclear techniques, 1983, 06(1): 28-32,75,76.
DOI：

Xiangtong Lin, Shouzhen Deng, Weiren Zhang, et al. Enzymatic radioiodination of polypeptide hormones and their stability in storage[J]. Nuclear techniques, 1983, 06(1): 28-32,75,76. DOI：

摘要

作者们自1978年起迄今三年间先后完成了HTSH、HGH、HCG、HLH、和OFSH等五种多肽激素的乳过氧化物酶（

Lactoperoxidase

，简称LPO）酶促

125

碘化法，结合国内目前供应的

125

碘化钠（含还原剂）的具体条件，选择酶促

125

碘化的最佳条件；酶及其组分内杂蛋白的自身

125

碘化问题；并对酶促法与氯胺T法制备的多肽激素的保存稳定性进行了对比研究，现总结如后。

Abstract

In this paper

enzymatic radioiodination of five polypeptide hormones (HTSH

HGH

HCG

HLH

OFSH) accomplished since 1978 is described studies were carried out in three respects: (1) The influance of various experimental conditions on enzymatic radioiodination

such as

amounts of LPO and H

pH value

time and temperature of reaction etc; (2)Stability of polypeptide hormones in storage and comparison between LPO method and CT method. It was shown that immunoactivity of former method is better and move persistant than that of the latter; (3) Beyond polypeptide hormones

radioiodination of LPO were studied. With practical amount of LPO

the iodination of LPO was trifle.

关键词

Keywords

references

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