A method for liquid scintillation counting of dual labelled samples and data handling with a desk-top computer

Faxian Chen; Junru Bao; Qing Shao

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A method for liquid scintillation counting of dual labelled samples and data handling with a desk-top computer

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- A method for liquid scintillation counting of dual labelled samples and data handling with a desk-top computer
- Nuclear Techniques Vol. 05, Issue 3, (1982)
- 作者机构：
  
  1.中国科学院上海生物化学研究所
  2.复旦大学
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Received：01 September 1980，
  
  Published：1982-06
- 稿件说明：
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Faxian Chen, Junru Bao, Qing Shao. A method for liquid scintillation counting of dual labelled samples and data handling with a desk-top computer[J]. Nuclear techniques, 1982, 05(3): 6-11.
DOI：

Faxian Chen, Junru Bao, Qing Shao. A method for liquid scintillation counting of dual labelled samples and data handling with a desk-top computer[J]. Nuclear techniques, 1982, 05(3): 6-11. DOI：

摘要

本文报导了

H和

P、

H和

C双标记样品液体闪烁测量法，使用仪器为Beckman LS8100和NE 8312液体闪烁谱仪。由双标记样品在仪器不同计数道中计数效率与

-数或外道比值

的关系，推导出计算双标记样品dpm的公式，所得数据用Wang 600型台式电子计算机处理。结果证明：这种测量和计算方法适用于双标记样品，并能得到满意的结果。

Abstract

This paper described a method in which

H and

C dual labelled samples were counted by a Beckman LS 8100 or an NE 8312 liquid scintillation spectrometer. An equation for calculating dpm of dual labelled samples was derived from the relation between the counting efficiency of dual labelled samples in different counting channels of a spectrometer and the H-number or external channel ratio value R. The data were processed by a Wang 600 calculator. The result shows that a satisfactory result can be obtained by using this method for measurement and calculation of dual labelled samples.

关键词

Keywords

references

R. DE Wachter and W. Fiers , Anal. Biochem. , 18 , 2 , 351 ( 1967 ).

T. C. Hall and C. J. Weiser , Anal. Biochem. , 17 , 294 ( 1966 ).

曹梅讯等，核技术， 1 , 64 （ 1978 ）.

E. J. C. Curtis and I. P. Toms , Liq. Scintill Counting , 2 , 167 ( 1971 ).

A. Dyer , An introduction to liq. scintill. counting , Heyden , London , 1974 , p. 69 .

A. G. Bruce and Olk C. Uhlenbeck , Nucleic Acids Research , 5 , 10 , 3665 ( 1978 ).

Donald L. Horrocks , Liq. Scintill. Counting , 5 , 145 ( 1977 ).

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