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淋巴细胞转化反应是体外测定机体免疫功能的常用方法,但在采用
3
H-TdR参入的淋巴细胞转化试验中,制样方法的不同,对结果的正确性影响很大。本文就消化法与滤膜法作了比较试验,还对不同规格的滤膜进行了筛选,现将结果报道如下。
During the experiment of lymphocyte transformation with
3
H-TdR
we found the counting rate of preparing sample using filter method was higher than that using chemical-digest method. Experiment showed the counting rate for 2
µ
Ci was doubled in filter method than digest method.
Moreover
when homemade 49 glass fiber disc was used in the filter method
the counting rate was markedly increased
in comparing with 0.8
μ
3μ
5μ millipore filter. The filtering rate was quite rapid and gave reproducible results. The liquid scintillator after filter disc being removed could be used over again.
杨希震 , 江苏省原子医学研究所情况交流 , 1979 .
B. K. Schrier et al ., Methods Cell Biology , 13 , 105 ( 1976 ).
中国科学院上海生物化学研究所四室 , 生物化学与生物物理进展 , 2 , 41 ( 1978 ).
中国科学院上海生物化学研究所四室 , 生物化学与生物物理进展 , 1 , 45 ( 1978 ).
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