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本文探讨用Na
125
I标记一些多肽激素时受氧化剂(氯胺
T
)用量、反应液pH、温度、体积和反应时间等因素的影响,标记化合物贮存时间长短对免疫活性反应的影响,以及贮存损伤等问题。
An oxidant (chloramine-
T
) on the reaction polypeptide hormones in labelling with Na
l25
I. The influence of dosage of oxidant (chloramine-
T
) volume
temperature and pH of the solution in labelling certain polypeptide hormones were investigated. The effect of storage time on immunological activity was also studied.
“放射免疫分析及其它体外测定方法”编辑组 , 放射免疫分析及其它体外测定方法 , 原子能出版社 , 1976 年.
F. C. Greenwood et al ., J. Biochem. , 89 , 114 ( 1963 ).
Goldfine et al ., J. Endocr. , 95 , 1228 ( 1974 ).
W. Charles and H. D. Parker , Radioimmunoassay of biologically active compounds Preofive-Hall , New Jersey , 1976 ,p. 68 .
M. Jacqueline , Clin. Chim. Acta , 70 , 157 ( 1976 ).
Ariel Gordin and Pikko Saarinen , Acta Endocrinology , 71 , 29 ( 1972 ).
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